Windarsih Anjar, Warmiko Hendy Dwi, Indrianingsih Anastasia Wheni, Rohman Abdul, Ulumuddin Yaya Ihya
Research Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia.
Research Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia.
Food Chem. 2022 Aug 30;386:132856. doi: 10.1016/j.foodchem.2022.132856. Epub 2022 Mar 29.
Pangasius hypopthalmus is well known as a good source of protein. However, Pangasius hypopthalmus meat (PHM) can be adulterated with pork for economic concern, thus, analytical methods for authentication are required. Untargeted metabolomics and proteomics using liquid chromatography-high resolution mass spectrometry (LC-HRMS) and chemometrics of principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) was successfully used to differentiate authentic and adulterated PHM with the good of fitness (R > 0.95) and good of predictivity (Q > 0.5). Metabolites of PC(o-18:0/18:2(9Z,12Z)) was found to be a potential marker for pork whereas DMPC (dimyristoylphosphatidylcholine) was a potential marker for PHM. Meanwhile, pork peptide marker of myoglobin (HPGDFGADAQGAMSK) and β-hemoglobin (FFESFGDLSNADAVMGNPK) could be identified. Both metabolomics and proteomics using LC-HRMS could detect pork at the lowest concentration level (0.5% w/w). In conclusion, untargeted metabolomics and proteomics using LC-HRMS in combination with chemometrics could be used as powerful methods to detect pork adulteration in fish meat.
巴沙鱼是众所周知的优质蛋白质来源。然而，出于经济考虑，巴沙鱼肉可能会被掺入猪肉，因此，需要用于鉴定的分析方法。使用液相色谱 - 高分辨率质谱（LC - HRMS）以及主成分分析（PCA）和偏最小二乘判别分析（PLS - DA）的化学计量学进行的非靶向代谢组学和蛋白质组学分析成功地用于区分纯正和掺假的巴沙鱼肉，拟合优度良好（R > 0.95）且预测性良好（Q > 0.5）。发现PC（o - 18:0/18:2(9Z,12Z)）的代谢物是猪肉的潜在标志物，而二肉豆蔻酰磷脂酰胆碱（DMPC）是巴沙鱼肉的潜在标志物。同时，可以鉴定出肌红蛋白（HPGDFGADAQGAMSK）和β - 血红蛋白（FFESFGDLSNADAVMGNPK）的猪肉肽标志物。使用LC - HRMS的代谢组学和蛋白质组学都可以检测到最低浓度水平（0.5% w/w）的猪肉。总之，使用LC - HRMS结合化学计量学的非靶向代谢组学和蛋白质组学可以作为检测鱼肉中猪肉掺假的有效方法。
