Department of Biomedical Engineering, National Cheng Kung University, Taiwan.
Department of Mechanical and Aerospace Engineering, North Carolina State University, USA.
Ultrasonics. 2022 Aug;124:106739. doi: 10.1016/j.ultras.2022.106739. Epub 2022 Mar 29.
Motor neuron diseases (MND) including amyotrophic lateral sclerosis and Parkinson disease are commonly neurodegenerative, causing a gradual loss of nerve cells and affecting the mechanisms underlying changes in calcium (Ca)-regulated dendritic growth. In this study, the NSC-34 cell line, a population of hybridomas generated using mouse spinal cord cells with neuroblastoma, was used to investigate the effect of low-intensity pulsed ultrasound (LIPUS) as part of an MND treatment model. After NSC-34 cells were seeded for 24 h, LIPUS stimulation was performed on the cells at days 1 and 3 using a non-focused transducer at 1.15 MHz for 8 min. NSC-34 cell proliferation and morphological changes were observed at various LIPUS intensities and different combinations of Ca channel blockers. The nuclear translocation of Ca-dependent transcription factors was also examined. We observed that the neurite outgrowth and cell number of NSC-34 significantly increased with LIPUS stimulation at days 2 and 4, which may be associated with the treatment's positive effect on the activation of Ca-dependent transcription factors, such as nuclear factor of activated T cells and nuclear factor-kappa B. Our findings suggest that the LIPUS-induced Ca signaling and transcription factor activation facilitate the morphological maturation and proliferation of NSC-34 cells, presenting a promising noninvasive method to improve stimulation therapy for MNDs in the future.
运动神经元病（MND）包括肌萎缩侧索硬化症和帕金森病，通常是神经退行性疾病，导致神经细胞逐渐丧失，并影响钙（Ca）调节树突生长的机制。在这项研究中，使用由鼠脊髓细胞与成神经细胞瘤生成的杂交瘤群体 NSC-34 细胞系来研究低强度脉冲超声（LIPUS）作为 MND 治疗模型的一部分的效果。在 NSC-34 细胞接种 24 小时后，在第 1 天和第 3 天使用非聚焦换能器在 1.15 MHz 下对细胞进行 8 分钟的 LIPUS 刺激。观察不同 LIPUS 强度和不同 Ca 通道阻滞剂组合对 NSC-34 细胞增殖和形态变化的影响。还检查了 Ca 依赖性转录因子的核易位。我们观察到，在 LIPUS 刺激下，NSC-34 的神经突生长和细胞数量在第 2 天和第 4 天显著增加，这可能与治疗对 Ca 依赖性转录因子（如激活 T 细胞的核因子和核因子 -kappa B）的激活的积极影响有关。我们的研究结果表明，LIPUS 诱导的 Ca 信号和转录因子激活促进了 NSC-34 细胞的形态成熟和增殖，为未来改善 MND 刺激治疗提供了一种有前途的非侵入性方法。
