Cheng Hsiao-Fan, Chiu Wen-Tai, Lai Yi-Shyun, Truong Thi-Thuyet, Lee Po-Yang, Huang Chih-Chung
Department of Biomedical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Department of Biomedical Engineering, National Cheng Kung University, Tainan 70101, Taiwan; Medical Device Innovation Center, National Cheng Kung University, Tainan 70101, Taiwan.
Ultrasonics. 2023 Jan;127:106852. doi: 10.1016/j.ultras.2022.106852. Epub 2022 Sep 27.
Chronic wounds have negative physical and psychological effects on patients and increase the health care burden. Consequently, chronic wound in the elderly population is an important issue. Ultrasound can be a great modality for treating chronic wounds because of its noninvasive and safety characteristics; it can accelerate in vitro and in vivo wound healing. In this study, we developed a novel noncontact ultrasound for wound treatment. We stimulated human epidermal keratinocyte migration using low-intensity pulsed ultrasound (LIPUS) with a noncontact transducer to avoid direct contact with the wound. We also compared the effects of 15-min contact and noncontact transducer stimulation, where a 1-MHz contact transducer (intensity = 40 or 200 mW/cm) and a 0.45-MHz noncontact transducer (intensity = 30 mW/cm) were used. Both contact and noncontact LIPUS considerably increased cell migration and activated the calcium (Ca)-dependent transcription factors cAMP-responsive element-binding protein (CREB) and nuclear factor of activated T cells (NFAT). Furthermore, noncontact transducer stimulation did not cause cell death or affect cell proliferation but significantly increased the Ca influx-mediated intracellular Ca levels. Ca-free medium and Ca channel blockers effectively inhibited LIPUS-induced Ca-dependent transcription factor activation and cell migration.
慢性伤口会对患者产生负面的生理和心理影响，并增加医疗负担。因此，老年人群中的慢性伤口是一个重要问题。由于超声具有非侵入性和安全性的特点，它可以成为治疗慢性伤口的一种很好的方式；它可以加速体外和体内的伤口愈合。在本研究中，我们开发了一种用于伤口治疗的新型非接触式超声。我们使用非接触式换能器通过低强度脉冲超声（LIPUS）刺激人表皮角质形成细胞迁移，以避免与伤口直接接触。我们还比较了15分钟接触式和非接触式换能器刺激的效果，其中使用了1-MHz的接触式换能器（强度 = 40或200 mW/cm）和0.45-MHz的非接触式换能器（强度 = 30 mW/cm）。接触式和非接触式LIPUS均显著增加了细胞迁移，并激活了钙（Ca）依赖性转录因子环磷酸腺苷反应元件结合蛋白（CREB）和活化T细胞核因子（NFAT）。此外，非接触式换能器刺激不会导致细胞死亡或影响细胞增殖，但会显著增加钙内流介导的细胞内钙水平。无钙培养基和钙通道阻滞剂有效抑制了LIPUS诱导的钙依赖性转录因子激活和细胞迁移。
