大肠杆菌谷氨酰胺合成酶基因（ntrB）产物氮调节蛋白II的纯化
Purification of nitrogen regulator II, the product of the glnL (ntrB) gene of Escherichia coli.
作者信息
Ninfa A J, Ueno-Nishio S, Hunt T P, Robustell B, Magasanik B
出版信息
J Bacteriol. 1986 Nov;168(2):1002-4. doi: 10.1128/jb.168.2.1002-1004.1986.
Abstract
We purified the product of glnL (ntrB), NRII, and the product of a mutant glnL allele, NRII2302. In vitro transcription of the nitrogen-regulated promoter glnAp2 by purified components of Escherichia coli required NRII or NRII2302 when the template DNA was linear.
摘要
我们纯化了谷氨酰胺合成酶基因L（ntrB）的产物NRII，以及谷氨酰胺合成酶基因L突变等位基因的产物NRII2302。当模板DNA呈线性时，利用大肠杆菌的纯化成分对氮调节启动子谷氨酰胺合成酶基因A2（glnAp2）进行体外转录需要NRII或NRII2302。
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