Matsumoto K, Uno I, Ishikawa T
J Bacteriol. 1986 Dec;168(3):1352-7. doi: 10.1128/jb.168.3.1352-1357.1986.
An fls1 mutant of Saccharomyces cerevisiae, which did not grow in the presence of 30 micrograms of fluphenazine per ml, was isolated. Mutants that were resistant to 90 micrograms of fluphenazine per ml and temperature sensitive for growth were obtained from the fls1 mutant. One fluphenazine-resistance mutation, fsr1, was located near the his7 locus on chromosome II. Growth of the fsr1 mutants at 35 degrees C was arrested after nuclear division. The other group of fluphenazine-resistant mutants, carrying fsr2 mutations, showed Ca2+-dependent growth at 35 degrees C. Growth of the fsr2 mutants at 35 degrees C was arrested at the G2 stage of the cell cycle in Ca2+-poor medium.
分离出了酿酒酵母的一个fls1突变体，该突变体在每毫升含30微克氟奋乃静的情况下无法生长。从fls1突变体中获得了对每毫升90微克氟奋乃静具有抗性且对生长温度敏感的突变体。一个氟奋乃静抗性突变fsr1位于第二条染色体上的his7基因座附近。fsr1突变体在35℃下生长，在核分裂后生长停滞。另一组携带fsr2突变的氟奋乃静抗性突变体在35℃下表现出Ca2 +依赖性生长。在Ca2 +缺乏的培养基中，fsr2突变体在35℃下的生长在细胞周期的G2期停滞。
