Farías R N, Viñals A L, Morero R D
J Biol Chem. 1986 Nov 25;261(33):15508-12.
We have studied, by fluorescence methods, the association of insulin to liposomes, the modification of lipid fluidity, and the fusion of vesicles induced by insulin. All parameters showed a similar dependence on pH and ionic strength of the medium and on negative charges in liposomes. The influence of temperature indicated that the association of insulin to liposomes per se was not sufficient to produce a decrease in lipid fluidity and fusion of liposomes. The modification of lipid fluidity induced by insulin in biological membranes is discussed as a possible general event in the action of the hormone.
我们通过荧光方法研究了胰岛素与脂质体的结合、脂质流动性的改变以及胰岛素诱导的囊泡融合。所有参数对培养基的pH值、离子强度以及脂质体中的负电荷均表现出相似的依赖性。温度的影响表明，胰岛素与脂质体本身的结合不足以导致脂质流动性降低和脂质体融合。胰岛素在生物膜中诱导的脂质流动性改变被作为该激素作用中可能的普遍事件进行了讨论。
