Farías R N, Viñals A L, Morero R D
Biochem Biophys Res Commun. 1985 Apr 16;128(1):68-74. doi: 10.1016/0006-291x(85)91645-6.
Fusion of negatively charged phospholipid vesicles by bovine insulin was studied. The fusion induced by the hormone was demonstrated by resonance energy transfer, sepharose chromatography, light scattering and electron microscopy. The insulin effect was more effective when the pH was in the range of 3.6 - 3.9. The action of insulin also depends on the phosphatidylcholine: phosphatidic acid molar ratio, and buffer and vesicles concentration. At optimal conditions, half-maximal effect was obtained at 2 X 10(-8)M. The insulin-mediated fusion is non specific. The potential importance of these studies is discussed.
研究了牛胰岛素对带负电荷磷脂囊泡的融合作用。通过共振能量转移、琼脂糖色谱、光散射和电子显微镜证实了该激素诱导的融合。当pH值在3.6 - 3.9范围内时，胰岛素的作用更有效。胰岛素的作用还取决于磷脂酰胆碱与磷脂酸的摩尔比、缓冲液和囊泡浓度。在最佳条件下，2×10(-8)M时可获得半数最大效应。胰岛素介导的融合是非特异性的。讨论了这些研究的潜在重要性。
