Pelzer H, Heimburger N
J Biomed Mater Res. 1986 Nov-Dec;20(9):1401-9. doi: 10.1002/jbm.820200913.
The blood compatibility of the primary reference materials, low density polyethylene and polydimethylsiloxane, was evaluated using human in vitro and human ex vivo test devices, and was compared with that of polyetherurethane and polyvinylchloride. The effect of the materials on in vitro platelet activation was studied by measurement of platelet factor 4 release by enzyme immunoassay. The adsorption of fibrinogen and platelets from human native blood onto the surface of the material was measured using enzyme immunoassay technique. The four materials tested exhibited significantly different effects on in vitro platelet activation. In addition, the materials adsorbed fibrinogen and platelets to a different extent under ex vivo test conditions. Materials, which induced strong in vitro platelet activation, e.g. low density polyethylene and polyvinylchloride, demonstrated high concentrations of fibrinogen and platelets on the surface when tested under ex vivo conditions. Polydimethylsiloxane and polyetherurethane, which induced slight in vitro platelet factor 4 release, absorbed significantly lower concentrations of fibrinogen and platelets from human native blood.
使用人体体外和人体离体测试装置对主要参考材料低密度聚乙烯和聚二甲基硅氧烷的血液相容性进行了评估，并与聚醚聚氨酯和聚氯乙烯的血液相容性进行了比较。通过酶免疫测定法测量血小板因子4的释放，研究了这些材料对体外血小板活化的影响。使用酶免疫测定技术测量人天然血液中纤维蛋白原和血小板在材料表面的吸附情况。测试的这四种材料对体外血小板活化表现出显著不同的影响。此外，在离体测试条件下，这些材料对纤维蛋白原和血小板的吸附程度也有所不同。在体外能强烈诱导血小板活化的材料，如低密度聚乙烯和聚氯乙烯，在离体条件下测试时，其表面显示出高浓度的纤维蛋白原和血小板。而聚二甲基硅氧烷和聚醚聚氨酯，它们在体外仅诱导轻微的血小板因子4释放，从人天然血液中吸附的纤维蛋白原和血小板浓度明显较低。
