Shehzad Aamir, Masud Awais, Fatima Tabassam, Khan Fraz Munir, Rehman Saifur, Effendi Mustofa Helmi, Suwanti Lucia Tri, Khan Iahtasham, Tyasningsih Wiwiek, Faisal Shah, Abadeen Zain Ul, Bibi Samreen
Division of Microbiology, Faculty of Veterinary Medicine, University of Airlangga, Surabaya 60115, Indonesia.
Livestock and Dairy Development, Government of Punjab, Lahore, Pakistan.
Vet World. 2022 Jan;15(1):110-118. doi: 10.14202/vetworld.2022.110-118. Epub 2022 Jan 23.
is an intracellular protozoan that infects humans and animals. This study aimed to estimate the seroprevalence of and the associated alterations in hematology and serum biochemistry of one-humped camels () in Mianwali district, Pakistan.
A total of 350 blood samples were obtained from male and female camels of different ages (≤3 years old, 4-6 years old, and ≥7 years old). To validate antibodies, the collected samples were subjected to indirect enzyme-linked immunosorbent assay using purified recombinant micronemal protein 3 as an antibody catching antigen.
The prevalence of was 50.2% higher in male camels than in female camels (16.5%) (p<0.001). Furthermore, the prevalence of in camels was directly proportional to age (p<0.001). It was 63.33% (57/90) in camels of ≥7 years of age, 32.54% in 4-6 years old age group, and 23.08% in ≤3 years old age group. The hematological analysis of infected camels revealed a significant increase in the values of glucocorticoid-remediable aldosteronism, lymphocyte percentage, monocyte percentage (MONO%), corpuscular hemoglobin (MCH), and procalcitonin. Furthermore, substantially higher levels of liver enzymes alanine aminotransferase, aspartate aminotransferase, and the macro-mineral potassium were found in the serum of -infected camels.
The seropositivity of is directly associated with the age and sex of camels, which may be considered as potential risk factors. Furthermore, infection directly impacts the hemato-biochemistry of infected camels.
[病原体名称]是一种感染人类和动物的细胞内原生动物。本研究旨在估计巴基斯坦米安瓦利地区单峰骆驼（[骆驼品种名称]）感染该病原体的血清阳性率以及相关的血液学和血清生化变化。
共采集了来自不同年龄（≤3岁、4 - 6岁和≥7岁）的雄性和雌性骆驼的350份血液样本。为验证[病原体名称]抗体，使用纯化的重组微小膜泡蛋白3作为抗体捕获抗原，对采集的样本进行间接酶联免疫吸附测定。
雄性骆驼中[病原体名称]的感染率比雌性骆驼（16.5%）高50.2%（p<0.001）。此外，骆驼中[病原体名称]的感染率与年龄成正比（p<0.001）。≥7岁的骆驼中感染率为63.33%（57/90），4 - 6岁年龄组为32.54%，≤3岁年龄组为23.08%。对感染骆驼的血液学分析显示，糖皮质激素可纠正性醛固酮增多症、淋巴细胞百分比、单核细胞百分比（MONO%）、平均红细胞血红蛋白含量（MCH）和降钙素原的值显著增加。此外，在感染[病原体名称]的骆驼血清中发现谷丙转氨酶、谷草转氨酶和常量矿物质钾的水平明显更高。
[病原体名称]的血清阳性率与骆驼的年龄和性别直接相关，这可能被视为潜在风险因素。此外，[病原体名称]感染直接影响感染骆驼的血液生化指标。
