Joint International Research Laboratory of Reproduction and Development, Chongqing Medical University Chongqing, PR China; Department of Reproductive Sciences, School of Public Health, Chongqing Medical University Chongqing, PR China.
Joint International Research Laboratory of Reproduction and Development, Chongqing Medical University Chongqing, PR China; Department of Physiology, School of Basic Medicine, Chongqing Medical University Chongqing, PR China.
Reprod Biomed Online. 2022 May;44(5):791-802. doi: 10.1016/j.rbmo.2021.10.024. Epub 2021 Nov 11.
What is the expression pattern of inflammatory mRNA profiles of a dehydroepiandrosterone (DHEA) plus high-fat diet (HFD)-induced polycystic ovary syndrome (PCOS) mouse model?
RNA sequencing was performed to investigate the mRNA expression profiles in the ovarian tissues of a DHEA plus HFD-induced PCOS mouse model. Six samples were divided into two groups (control and PCOS), with three biological replicates in each group. This was followed by hierarchical clustering, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. The relative expression levels of nine inflammatory genes were validated via quantitative reverse-transcription polymerase chain reaction.
A total of 436 genes were differentially expressed between the control and PCOS mice. Out of these, 137 genes were up-regulated while 299 genes were down-regulated. Gene ontology analysis indicated that differentially expressed mRNA were associated with T cell-mediated cytotoxicity and homocysteine metabolic processes. Pathway analysis further showed that these abnormally expressed mRNA were associated with signalling pathways, such as NF-kB signalling, tyrosine metabolism and phenylalanine metabolism. All these pathways are involved in chronic inflammation and PCOS.
The differentially expressed genes are potentially involved in the inflammation that is evident in PCOS, and so could serve as therapeutic options against the disease. Nevertheless, prospective studies are needed to test this hypothesis.
脱氢表雄酮（DHEA）加高脂饮食（HFD）诱导的多囊卵巢综合征（PCOS）小鼠模型的炎症 mRNA 谱表达模式是什么？
通过 RNA 测序研究 DHEA 加 HFD 诱导的 PCOS 小鼠模型卵巢组织中的 mRNA 表达谱。将六个样本分为两组（对照组和 PCOS 组），每组三个生物学重复。然后进行层次聚类、基因本体论和京都基因与基因组百科全书通路分析。通过定量逆转录聚合酶链反应验证了 9 个炎症基因的相对表达水平。
对照组和 PCOS 小鼠之间共有 436 个基因表达差异。其中，137 个基因上调，299 个基因下调。基因本体论分析表明，差异表达的 mRNA 与 T 细胞介导的细胞毒性和同型半胱氨酸代谢过程有关。通路分析进一步表明，这些异常表达的 mRNA 与信号通路有关，如 NF-kB 信号通路、酪氨酸代谢和苯丙氨酸代谢。所有这些途径都与慢性炎症和 PCOS 有关。
差异表达的基因可能与 PCOS 中明显的炎症有关，因此可以作为治疗该疾病的选择。然而，需要前瞻性研究来验证这一假设。
