Kopp W, Marggraf E
J Clin Periodontol. 1986 Oct;13(9):805-9. doi: 10.1111/j.1600-051x.1986.tb02234.x.
Peripheral blood lymphocytes were obtained from 10 patients with advanced periodontitis and from 5 normal control subjects. The lymphocytes were cultured with dental plaque, supernatant of homologous fibroblast cytoplasm and Pokeweed-mitogen (PWM). The number of immunoglobulin-secreting cells (ISC) was assessed after 7 days using a reverse hemolytic plaque assay (RHPA). The stimulation levels of lymphocytes from periodontitis patients and control subjects stimulated with dental plaque and PWM were not statistically different (p greater than 0.05). In contrast, the number of ISC produced after stimulation with fibroblast cytoplasm was increased significantly in the patients group (p less than 0.05). These data support the hypothesis that autologous substances play an important rôle in the cellular immune responses of patients with severe chronic inflammatory periodontal disease (CIPD).
从10例晚期牙周炎患者和5名正常对照受试者中获取外周血淋巴细胞。将淋巴细胞与牙菌斑、同源成纤维细胞胞质上清液和商陆有丝分裂原（PWM）一起培养。7天后，使用反向溶血空斑试验（RHPA）评估免疫球蛋白分泌细胞（ISC）的数量。牙菌斑和PWM刺激的牙周炎患者和对照受试者的淋巴细胞刺激水平无统计学差异（p>0.05）。相比之下，成纤维细胞胞质刺激后产生的ISC数量在患者组中显著增加（p<0.05）。这些数据支持以下假设：自体物质在重度慢性炎症性牙周病（CIPD）患者的细胞免疫反应中起重要作用。
