Lanzavecchia A, Sacchi G, Maccario R, Vitiello A, Nespoli L, Ugazio A G
Scand J Immunol. 1979;10(4):297-302. doi: 10.1111/j.1365-3083.1979.tb01354.x.
Cells secreting IgM, IgG and IgA were evaluated in cultures of human peripheral blood lymphocytes stimulated in vitro with pokeweed mitogen by a haemolytic plaque assay using protein A-coated erythrocytes in the presence of class-specific antisera. Kinetic study revealed that immunoglobulin-secreting cells appeared after 3 days of culture and peaked between days 5 and 7. IgM-secreting cells predominated throughout the culture period. This plaque assay is a useful and sensitive in vitro test for the evaluation of polyclonal B-cell activation in humans and may provide a good approach to the study of disturbances in the synthesis of the various classes of immunoglobulins.
运用蛋白A包被的红细胞，在类特异性抗血清存在的情况下，通过溶血空斑试验，对用商陆丝裂原体外刺激的人外周血淋巴细胞培养物中分泌IgM、IgG和IgA的细胞进行了评估。动力学研究显示，免疫球蛋白分泌细胞在培养3天后出现，并在第5至7天达到峰值。在整个培养期间，分泌IgM的细胞占主导地位。这种空斑试验是评估人类多克隆B细胞活化的一种有用且灵敏的体外试验，可能为研究各类免疫球蛋白合成紊乱提供一个良好的方法。
