Kurtz M E, Martin-Morgan D, Graff R J
J Immunol. 1987 Jan 1;138(1):87-90.
In this report we offer evidence that the beta-2 microglobulin-B (beta 2M-B) molecule is recognized by a cytotoxic T lymphocyte (CTL). Production of GA5, a CTL clone reactive against a membrane antigen with the same strain distribution pattern as beta 2M-B, is described. This clone lysed syngeneic target cells in which native beta 2M-A molecules had been exchanged with beta 2M-B molecules by incubating the cells in serum from beta 2M-B-positive mouse strains. Conversely, the CTL clone GA5 failed to lyse its specific target when beta 2M-B molecules had been exchanged with beta 2M-A molecules by incubating the cells in serum from beta 2M-A-positive, beta 2M-B-negative mouse strains. Strain combinations were chosen so as to limit reactivity to beta 2M and to preclude reactivity to H-3 antigens, thus indicating CTL clone GA5 to be reacting specifically with beta 2M-B.
在本报告中，我们提供证据表明β2微球蛋白-B（β2M-B）分子可被细胞毒性T淋巴细胞（CTL）识别。本文描述了GA5的产生，GA5是一种CTL克隆，它对一种膜抗原具有反应性，该膜抗原与β2M-B具有相同的菌株分布模式。通过在来自β2M-B阳性小鼠品系的血清中培养细胞，该克隆裂解了同基因靶细胞，其中天然的β2M-A分子已被β2M-B分子所取代。相反，当通过在来自β2M-A阳性、β2M-B阴性小鼠品系的血清中培养细胞，使β2M-B分子被β2M-A分子取代时，CTL克隆GA5未能裂解其特异性靶细胞。选择品系组合是为了限制对β2M的反应性，并排除对H-3抗原的反应性，从而表明CTL克隆GA5与β2M-B发生特异性反应。
