Contreas G, Madsen O D, Vissing H, Lernmark A
J Immunol Methods. 1986 Dec 4;95(1):135-9. doi: 10.1016/0022-1759(86)90328-5.
A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.
已开发出一种用于检测胰岛细胞表面抗体（CIRMA）的简单灵敏的免疫放射分析方法。从可移植胰岛细胞瘤肝转移灶中获取的活的、转化的胰岛细胞在可拆卸的微量滴定孔中培养，并与抗体孵育。使用125I标记的二抗对细胞结合抗体进行定量。该分析方法用于检测非糖尿病和糖尿病BB大鼠血清中存在的胰岛细胞抗体，并且被证明对于筛选杂交瘤上清液以鉴定识别胰岛细胞表面抗原的单克隆抗体特别有效。
