Huen A H, Haneda M, Freedman Z, Lernmark A, Rubenstein A H
Diabetes. 1983 May;32(5):460-5. doi: 10.2337/diab.32.5.460.
A quantitative method to measure islet cell surface antibodies in human patients has been developed using 125I-protein A. Isolated, dispersed, viable rat islet cells prepared by collagenase digestion were fixed in 4% paraformaldehyde to allow storage for up to 7 wk at 4 degrees C. Human sera, heat inactivated and adsorbed with rat liver and kidney powder (100 mg/ml), were incubated with the fixed cells (50 x 10(3)) for 60 min at 37 degrees C. Thereafter the cells were washed and exposed to 5 x 10(5) cpm 125I-protein A, which binds to IgG attached to the cell surface. Assay precision (14%) and reproducibility (16%) were established by repeated analysis of pooled sera from healthy individuals and IDDM patients using pooled batches of islet cells. Using this method, islet cell surface antibodies were detected in 35% of insulin-dependent diabetic patients.
一种使用¹²⁵I - 蛋白A来测量人类患者胰岛细胞表面抗体的定量方法已被开发出来。通过胶原酶消化制备的分离、分散、有活力的大鼠胰岛细胞，用4%多聚甲醛固定，以便在4℃下储存长达7周。经过热灭活并用大鼠肝粉和肾粉（100mg/ml）吸附的人血清，与固定细胞（50×10³个）在37℃孵育60分钟。此后，细胞被洗涤并暴露于5×10⁵cpm的¹²⁵I - 蛋白A，该蛋白A会与附着在细胞表面的IgG结合。通过使用合并批次的胰岛细胞对健康个体和胰岛素依赖型糖尿病患者的合并血清进行重复分析，确定了检测精度（14%）和重现性（16%）。使用这种方法，在35%的胰岛素依赖型糖尿病患者中检测到了胰岛细胞表面抗体。
