Sontheimer R D, Stastny P, Nũnez G
J Invest Dermatol. 1986 Dec;87(6):707-10. doi: 10.1111/1523-1747.ep12456670.
We have systematically examined HLA-D region antigen expression by normal human epidermal Langerhans cells with an improved double label indirect immunofluorescence technique in order to better understand the immunologic potential of this human interstitial dendritic cell type. The results of this study which differ somewhat from earlier observations strongly suggest that 100% of OKT-6-positive epidermal Langerhans cells constitutively express HLA-DQ and HLA-DP as well as HLA-DR molecules. Since HLA-DQ expression has been positively correlated with the ability to present nominal antigen, our findings would suggest the possibility that epidermal Langerhans cells, like other members of the human dendritic cell series, might be more efficient than monocytes at nominal antigen presentation since only about 50% of peripheral blood monocytes express HLA-DQ molecules.
我们运用改良的双标记间接免疫荧光技术，系统地检测了正常人表皮朗格汉斯细胞的HLA - D区抗原表达情况，以便更好地了解这种人体间质树突状细胞类型的免疫潜能。本研究结果与早期观察结果略有不同，有力地表明100%的OKT - 6阳性表皮朗格汉斯细胞组成性表达HLA - DQ、HLA - DP以及HLA - DR分子。由于HLA - DQ表达与呈递名义抗原的能力呈正相关，我们的研究结果表明，表皮朗格汉斯细胞与人类树突状细胞系列的其他成员一样，在呈递名义抗原方面可能比单核细胞更有效，因为外周血单核细胞中只有约50%表达HLA - DQ分子。
