Barrett A W, Ross D A, Goodacre J A
Department of Oral Biology, University of Newcastle-upon-Tyne, UK.
Clin Exp Immunol. 1993 Apr;92(1):158-63. doi: 10.1111/j.1365-2249.1993.tb05963.x.
Oral mucosal Langerhans cells (OMLC) may have an important role in the induction of immune responses to oral pathogens. In this study, anti-HLA-DR antibody-coated immunomagnetic beads were used to purify OMLC from suspensions of normal human buccal epithelium and the capacity of the purified cells to function as accessory cells (AC) was investigated. Electron microscopy was used to show that the purified cells possessed all recognized ultrastructural features previously described in epidermal Langerhans cells. Using T lymphocyte proliferation assays in hanging drop microcultures, it was found that purified OMLC could function as AC for responses to concanavalin A by autologous T cells. Purification of OMLC from small biopsies of oral mucosa has enabled us to show that OMLC, like epidermal Langerhans cells, can function as AC in vitro.
口腔黏膜朗格汉斯细胞（OMLC）可能在诱导针对口腔病原体的免疫反应中发挥重要作用。在本研究中，使用抗HLA - DR抗体包被的免疫磁珠从正常人颊黏膜上皮悬液中纯化OMLC，并研究纯化细胞作为辅助细胞（AC）的功能。电子显微镜显示纯化的细胞具有先前在表皮朗格汉斯细胞中描述的所有公认的超微结构特征。利用悬滴微量培养中的T淋巴细胞增殖试验，发现纯化的OMLC可作为自体T细胞对刀豆球蛋白A反应的辅助细胞。从口腔黏膜小活检组织中纯化OMLC使我们能够证明，OMLC与表皮朗格汉斯细胞一样，在体外可作为辅助细胞发挥作用。
