Jung Hoseok, Seo Wookcheol, Jeong Taeseong, Kang Hyung Won, Kim Sungchul
Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital, Gwangju, Republic of Korea.
Department of Korean Neuropsychiatry Medicine & Inam Neuroscience Research Center, Wonkwang University Sanbon Hospital, Gunpo, Republic of Korea.
J Pharmacopuncture. 2022 Mar 31;25(1):46-51. doi: 10.3831/KPI.2022.25.1.46.
This study was performed to evaluate the skin irritation toxicity of processed sulfur.
All experiments were conducted at Medvill (Korea), an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate skin irritation toxicity of processed sulfur, we divided the back of six rabbits into two control sites and two test sites. One of each of the two control and test sites was then designated abraded sites and intact sites. In test sites, 0.5 g of processed sulfur was applied to the back of the rabbit for 24 hours, and in control sites, 0.5 g of sterile distilled water was applied in the same way. We observed and evaluated mortality, weight, general symptoms, and skin irritation toxicity. This study was conducted with the approval of the Animal Ethics Committee (Approval number IAC2020-1549).
In all experiments, no dead animals were observed. In all cases, skin coloration was observed at 24 hours after processed sulfur administration. This coloration lasted up to 48 hours and is believed to be the effect of the administration of test substances. Weight measurement indicated that weight was lost 72 hours after administration in three cases, but this is considered an accidental weight change. Normal weight gain was observed in the remaining subjects. In all animals, no skin irritation toxicity was observed, and the primary irritation index (P.I.I) was calculated as 0.0 according to Draize's evaluation method.
The above findings suggest that it is relatively safe to apply a processed sulfur to the skin. Further research on this topic is needed to provide more specific evidence.
本研究旨在评估精制硫磺的皮肤刺激毒性。
所有实验均在韩国Medvill进行，该机构是一家经授权进行非临床研究的机构，遵循良好实验室规范（GLP）法规。为了研究精制硫磺的皮肤刺激毒性，我们将六只兔子的背部划分为两个对照部位和两个测试部位。然后将两个对照部位和测试部位中的各一个指定为擦伤部位和完整部位。在测试部位，将0.5 g精制硫磺涂抹在兔子背部24小时，在对照部位，以相同方式涂抹0.5 g无菌蒸馏水。我们观察并评估了死亡率、体重、一般症状和皮肤刺激毒性。本研究经动物伦理委员会批准（批准号IAC2020 - 1549）。
在所有实验中，均未观察到动物死亡。在所有情况下，施用精制硫磺24小时后观察到皮肤着色。这种着色持续长达48小时，被认为是施用受试物质的效果。体重测量表明，有三只兔子在给药72小时后体重下降，但这被认为是偶然的体重变化。其余兔子体重正常增加。在所有动物中，均未观察到皮肤刺激毒性，根据Draize评估方法计算的原发性刺激指数（P.I.I）为0.0。
上述研究结果表明，将精制硫磺涂抹于皮肤相对安全。需要对该主题进行进一步研究以提供更具体的证据。
