Yin Wenhao, Wang Xinping, Liao Ying, Ma Lixin, Qiao Jie, Liu Hui, Song Xin, Liu Yi
School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China.
State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou University, Henan, China.
Front Bioeng Biotechnol. 2022 Mar 17;10:849542. doi: 10.3389/fbioe.2022.849542. eCollection 2022.
There are several commercial chromatographic systems for protein purification; however, development of cost-effective 3H-grade (high yield, high purity, and high activity) purification approaches is highly demanded. Here, we establish a methodology for encapsulating the IM7-displaying yeast cells in calcium alginate beads. Taking advantage of this biomaterial-based affinity chromatography, rapid and cost-effective purification of proteins with over 90% purity in a single step is achieved. Moreover, our system enables coating the multienzyme complex to produce reusable immobilized cells for efficient cascade biotransformation. Together, the present method has great application potentials not only in the laboratory but also in the industry for production of protein products as well as biocatalysis.
有几种用于蛋白质纯化的商业色谱系统；然而，人们迫切需要开发具有成本效益的3H级（高产率、高纯度和高活性）纯化方法。在此，我们建立了一种将展示IM7的酵母细胞包裹在海藻酸钙珠中的方法。利用这种基于生物材料的亲和色谱法，可在一步中快速且经济高效地纯化纯度超过90%的蛋白质。此外，我们的系统能够包被多酶复合物以生产可重复使用的固定化细胞用于高效级联生物转化。总之，本方法不仅在实验室而且在蛋白质产品生产以及生物催化的工业领域都具有巨大的应用潜力。
