Picker Jonathan, Lan Ziyang, Arora Srishtee, Green Mykel, Hahn Mariah, Cosgriff-Hernandez Elizabeth, Hook Magnus
Center for Infectious and Inflammatory Diseases, Institute of Biosciences and Technology, Texas A&M, Houston, TX, United States.
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, United States.
Front Bioeng Biotechnol. 2022 Mar 17;10:840939. doi: 10.3389/fbioe.2022.840939. eCollection 2022.
Collagens are the major structural component in animal extracellular matrices and are critical signaling molecules in various cell-matrix interactions. Its unique triple helical structure is enabled by tripeptide Gly-X-Y repeats. Understanding of sequence requirements for animal-derived collagen led to the discovery of prokaryotic collagen-like protein in the early 2000s. These prokaryotic collagen-like proteins are structurally similar to mammalian collagens in many ways. However, unlike the challenges associated with recombinant expression of mammalian collagens, these prokaryotic collagen-like proteins can be readily expressed in and are amenable to genetic modification. In this review article, we will first discuss the properties of mammalian collagen and provide a comparative analysis of mammalian collagen and prokaryotic collagen-like proteins. We will then review the use of prokaryotic collagen-like proteins to both study the biology of conventional collagen and develop a new biomaterial platform. Finally, we will describe the application of Scl2 protein, a streptococcal collagen-like protein, in thromboresistant coating for cardiovascular devices, scaffolds for bone regeneration, chronic wound dressing and matrices for cartilage regeneration.
胶原蛋白是动物细胞外基质中的主要结构成分，并且是各种细胞与基质相互作用中的关键信号分子。其独特的三螺旋结构由三肽Gly-X-Y重复序列形成。对动物源胶原蛋白序列要求的理解促使在21世纪初发现了原核生物胶原蛋白样蛋白。这些原核生物胶原蛋白样蛋白在结构上在许多方面与哺乳动物胶原蛋白相似。然而，与哺乳动物胶原蛋白重组表达相关的挑战不同，这些原核生物胶原蛋白样蛋白可以在（此处原文似乎缺失相关信息）中轻松表达并且易于进行基因修饰。在这篇综述文章中，我们将首先讨论哺乳动物胶原蛋白的特性，并对哺乳动物胶原蛋白和原核生物胶原蛋白样蛋白进行比较分析。然后，我们将综述原核生物胶原蛋白样蛋白在研究传统胶原蛋白生物学以及开发新的生物材料平台方面的应用。最后，我们将描述链球菌胶原蛋白样蛋白Scl2在心血管装置抗血栓涂层、骨再生支架、慢性伤口敷料和软骨再生基质中的应用。
