基于网络药理学探讨三七治疗心肌纤维化的作用机制。
Using Network Pharmacology to Explore the Mechanism of Panax notoginseng in the Treatment of Myocardial Fibrosis.
机构信息
Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China.
Heilongjiang Academy of Chinese Medical Sciences, Harbin 150036, China.
出版信息
J Diabetes Res. 2022 Mar 25;2022:8895950. doi: 10.1155/2022/8895950. eCollection 2022.
OBJECTIVE
The mechanism of Panax notoginseng in treating myocardial fibrosis (MF) was investigated using network pharmacology.
METHODS
Effective ingredients and potential targets of Panax notoginseng were screened in relevant databases to construct a compound-target network. Targets of MF were then screened to select common targets and construct a protein-protein interaction network. This was followed by Gene Ontology and pathway enrichment analyses. Molecular docking then verified the results of network analysis.
RESULTS
A total of 14 effective ingredients and 119 potential targets for MF were predicted. Quercetin, beta-sitosterol, and gossypetin were speculated to be the main active ingredients. The mechanism of action may be related to AGE-RAGE, proteoglycans, and IL-17 signaling pathways. Five key targets (IL6, ALB, AKT1, TNF, and VEGFA) may be involved in the treatment of MF using Panax notoginseng.
CONCLUSIONS
This study embodies the complex network relationship of multicomponents, multitargets, and multipathways of Panax notoginseng in treating MF and provides a novel method for further research on this herb's mechanism.
目的
采用网络药理学研究三七治疗心肌纤维化（MF）的作用机制。
方法
在相关数据库中筛选三七的有效成分和潜在靶点，构建化合物-靶点网络。然后筛选 MF 的靶点，选择共同靶点并构建蛋白质-蛋白质相互作用网络。接着进行基因本体和通路富集分析。最后通过分子对接验证网络分析的结果。
结果
共预测到 14 种有效成分和 119 个 MF 的潜在靶点。槲皮素、β-谷甾醇和棉子苷可能是主要的活性成分。作用机制可能与 AGE-RAGE、蛋白聚糖和 IL-17 信号通路有关。五个关键靶点（IL6、ALB、AKT1、TNF 和 VEGFA）可能参与了三七治疗 MF。
结论
本研究体现了三七多成分、多靶点、多途径治疗 MF 的复杂网络关系，为进一步研究该药物的作用机制提供了一种新方法。
Zotero 插件