Hsa_circ_0058129 通过 miR-873-5p/follistatin-like 1 轴调控甲状腺乳头状癌的发生发展。

Hsa_circ_0058129 regulates papillary thyroid cancer development via miR-873-5p/follistatin-like 1 axis.

机构信息

Head and Neck Surgery, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China.

出版信息

J Clin Lab Anal. 2022 May;36(5):e24401. doi: 10.1002/jcla.24401. Epub 2022 Apr 4.

BACKGROUND

Papillary thyroid cancer (PTC) is an endocrine malignancy with a high incidence. Circular RNAs (circRNAs) participate in regulating PTC. Here, we analyzed the role of hsa_circ_0058129 (circ_0058129) in PTC.

METHODS

The expression of circ_0058129, fibronectin 1 (FN1) mRNA, microRNA-873-5p (miR-873-5p), and follistatin-like 1 (FSTL1) was detected by qRT-PCR and western blot. Cell proliferation was analyzed by CCK-8, EdU, and flow cytometry analysis assays. Cell migration and invasion were evaluated by Transwell assay. The targeting relationship of miR-873-5p and circ_0058129 or FSTL1 was identified through dual-luciferase reporter assay, RIP assay, and RNA pull-down assay. Xenograft mouse model assay was implemented to determine the effect of circ_0058129 on tumor formation in vivo.

RESULTS

The circ_0058129 and FSTL1 abundances were increased, while the miR-873-5p content was decreased in PTC tissues and cells compared with control groups. Circ_0058129 shortage inhibited PTC cell proliferation, migration, and invasion. Moreover, miR-873-5p repressed PTC cell malignancy by binding to FSTL1. Circ_0058129 targeted miR-873-5p to regulate FSTL1.

CONCLUSION

Circ_0058129 expedited PTC progression through the miR-873-5p/FSTL1 pathway.

背景

甲状腺乳头状癌（PTC）是一种发病率较高的内分泌恶性肿瘤。环状 RNA（circRNA）参与调节 PTC。在这里，我们分析了 hsa_circ_0058129（circ_0058129）在 PTC 中的作用。

方法

通过 qRT-PCR 和 Western blot 检测 circ_0058129、纤维连接蛋白 1（FN1）mRNA、微小 RNA-873-5p（miR-873-5p）和卵泡抑素样 1（FSTL1）的表达。通过 CCK-8、EdU 和流式细胞术分析检测细胞增殖。通过 Transwell 测定评估细胞迁移和侵袭。通过双荧光素酶报告基因测定、RIP 测定和 RNA 下拉测定鉴定 miR-873-5p 和 circ_0058129 或 FSTL1 的靶向关系。通过异种移植小鼠模型实验确定 circ_0058129 对体内肿瘤形成的影响。

结果

与对照组相比，PTC 组织和细胞中的 circ_0058129 和 FSTL1 丰度增加，而 miR-873-5p 含量降低。circ_0058129 缺乏抑制 PTC 细胞增殖、迁移和侵袭。此外，miR-873-5p 通过与 FSTL1 结合抑制 PTC 细胞恶性转化。circ_0058129 靶向 miR-873-5p 以调节 FSTL1。