Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China.
Jiangxi Key Laboratory for Individualized Cancer Therapy, Nanchang, Jiangxi, P.R. China.
World J Surg Oncol. 2020 Dec 8;18(1):324. doi: 10.1186/s12957-020-02070-9.
Follistatin-like 1 (FSTL1) plays a central role in the progression of tumor and tumor immunity. However, the effect of FSTL1 on the prognosis and immune infiltration of gastric cancer (GC) remains to be elucidated.
The expression of FSTL1 data was analyzed in Oncomine and TIMER databases. Analyses of clinical parameters and survival data were conducted by Kaplan-Meier plotter and immunohistochemistry. Western blot assay and real-time quantitative PCR (RT-qPCR) were used to analyze protein and mRNA expression, respectively. The correlations between FSTL1 and cancer immune infiltrates were analyzed by Tumor Immune Estimation Resource (TIME), Gene Expression Profiling Interactive Analysis (GEPIA), and LinkedOmics database.
The expression of FSTL1 was significantly higher in GC tissues than in normal tissues, and bioinformatic analysis and immunohistochemistry (IHC) indicated that high FSTL1 expression significantly correlated with poor prognosis in GC. Moreover, FSTL1 was predicted as an independent prognostic factor in GC patients. Bioinformatics analysis results suggested that FSTL1 mainly involved in tumor progression and tumor immunity. And significant correlations were found between FSTL1 expression and immune cell infiltration in GC.
The study effectively revealed useful information about FSTL1 expression, prognostic values, potential functional networks, and impact of tumor immune infiltration in GC. In summary, FSTL1 can be used as a biomarker for prognosis and evaluating immune cell infiltration in GC.
卵泡抑素样蛋白 1（Follistatin-like 1，FSTL1）在肿瘤的发生和肿瘤免疫中起着核心作用。然而，FSTL1 对胃癌（GC）的预后和免疫浸润的影响仍有待阐明。
在 Oncomine 和 TIMER 数据库中分析 FSTL1 的表达数据。通过 Kaplan-Meier 绘图器和免疫组织化学分析临床参数和生存数据。Western blot 检测和实时定量 PCR（RT-qPCR）分别用于分析蛋白和 mRNA 表达。通过肿瘤免疫估计资源（Tumor Immune Estimation Resource，TIME）、基因表达谱交互式分析（Gene Expression Profiling Interactive Analysis，GEPIA）和 LinkedOmics 数据库分析 FSTL1 与癌症免疫浸润的相关性。
GC 组织中 FSTL1 的表达明显高于正常组织，生物信息学分析和免疫组织化学（IHC）表明，FSTL1 高表达与 GC 的不良预后显著相关。此外，FSTL1 被预测为 GC 患者的独立预后因素。生物信息学分析结果表明，FSTL1 主要参与肿瘤进展和肿瘤免疫。并且在 GC 中发现 FSTL1 表达与免疫细胞浸润之间存在显著相关性。
本研究有效地揭示了 FSTL1 在 GC 中的表达、预后价值、潜在功能网络以及对肿瘤免疫浸润的影响的有用信息。总之，FSTL1 可以作为 GC 预后和评估免疫细胞浸润的标志物。
