Kuhn A, Keller B, Maeder M, Traub F
J Virol. 1987 Jan;61(1):113-8. doi: 10.1128/JVI.61.1.113-118.1987.
Bacteriophage T4 assembly was impaired in Escherichia coli hdB3-1 at an incubation temperature below 30 degrees C. Naked prohead cores (head scaffold) bound to the inner surface of the plasma membrane accumulated, and the major shell protein (gp23) precipitated into visible intracellular aggregates in the cytoplasm. Shifting the temperature to 42 degrees C allowed newly synthesized gp23 to assemble around the accumulated cores. We conclude that synchronous assembly of the scaffold and shell is not obligatory and that naked cores can serve as intermediates in the T4 assembly pathway.
在低于30摄氏度的培养温度下，大肠杆菌hdB3-1中噬菌体T4的组装受到损害。与质膜内表面结合的裸露前头部核心（头部支架）积累，并且主要外壳蛋白（gp23）沉淀到细胞质中可见的细胞内聚集体中。将温度转移到42摄氏度可使新合成的gp23围绕积累的核心组装。我们得出结论，支架和外壳的同步组装不是必需的，并且裸露的核心可以作为T4组装途径中的中间体。
