用N-甲基-N-亚硝基脲和硫酸二甲酯对聚（dA-dT）进行甲基化时O2-甲基胸腺嘧啶的形成。O2-甲基胸腺嘧啶在DNA合成过程中不会发生错编码的证据。
Formation of O2-methylthymine in poly(dA-dT) on methylation with N-methyl-N-nitrosourea and dimethyl sulphate. Evidence that O2-methylthymine does not miscode during DNA synthesis.
作者信息
Saffhill R, Abbott P J
出版信息
Nucleic Acids Res. 1978 Jun;5(6):1971-8. doi: 10.1093/nar/5.6.1971.
Abstract
The alternating co-polymer has been methylated with either N methyl-N-nitrosourea (MNU) or dimethyl sulphate (DMS) and the levels of the various methylated thymidines (O2-methylthymidine, 3-methylthymidine and O4-methylthymidine) measured. MNU produced all three compounds whereas DMS only produced 3-methylthymidine and O2-methylthymidine at detectable levels. These results have been combined with our earlier results concerning the misincorporation of dGMP with E. coli DNA polymerase using MNU-methylated poly(dA-dT). These results indicate that O2-methylthymidine does not miscode during DNA synthesis.
摘要
交替共聚物已用N-甲基-N-亚硝基脲（MNU）或硫酸二甲酯（DMS）进行甲基化，并测定了各种甲基化胸苷（O2-甲基胸苷、3-甲基胸苷和O4-甲基胸苷）的水平。MNU产生了所有三种化合物，而DMS仅产生了可检测水平的3-甲基胸苷和O2-甲基胸苷。这些结果与我们早期关于使用MNU甲基化的聚（dA-dT）时dGMP与大肠杆菌DNA聚合酶错误掺入的结果相结合。这些结果表明，O2-甲基胸苷在DNA合成过程中不会发生错误编码。
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