Department of Ophthalmology, Manhattan Eye, Ear, and Throat Hospital, Northwell Health, New York, NY, USA.
Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USA.
Doc Ophthalmol. 2022 Aug;145(1):11-25. doi: 10.1007/s10633-022-09869-9. Epub 2022 Apr 4.
To estimate retinal ganglion cell (RGC) count in glaucoma suspects (GS) and ascertain its relationships with steady-state pattern electroretinography (ssPERG) parameters.
In this prospective cross-sectional study, 22 subjects (44 eyes) were recruited at the Manhattan Eye, Ear, and Throat Hospital. Subjects underwent complete eye examinations, optical coherence tomography, standard automated perimetry, and ssPERG testing. Eyes were divided into two groups based upon clinical data: healthy subjects and GS. RGC count was estimated using the combined structure-function index.
Estimated RGC count, average retinal nerve fiber layer thickness (ARNFLT), and average ganglion cell layer and inner plexiform layer thickness (GCIPLT) were reduced in GS eyes (p ≤ 0.001 for all parameters). Pearson correlations revealed that ssPERG magnitude and magnitudeD correlated with ARNFLT (r ≥ 0.53, p < 0.001), GCIPLT (r > 0.38, p < 0.011), and estimated RGC count (r > 0.46, p < 0.002). Six mediation analyses revealed that estimated RGC count mediated the relationships among ssPERG parameters, ARNFLT, and GCIPLT.
Steady-state PERG parameters demonstrated linear correlations with estimated RGC count. The associations among ssPERG parameters and structural measures were mediated by estimated RGC count.
评估疑似青光眼（GS）患者的视网膜神经节细胞（RGC）计数，并确定其与稳态图形视觉诱发电位（ssPERG）参数的关系。
本前瞻性横断面研究共招募了 22 名受试者（44 只眼），他们均来自曼哈顿眼、耳和喉医院。所有受试者均接受了全面的眼部检查、光学相干断层扫描、标准自动视野检查和 ssPERG 测试。根据临床数据，将眼睛分为两组：健康受试者和 GS。使用联合结构-功能指数来估计 RGC 计数。
GS 眼的估计 RGC 计数、平均视网膜神经纤维层厚度（ARNFLT）和平均神经节细胞层和内丛状层厚度（GCIPLT）均降低（所有参数的 p 值均≤0.001）。Pearson 相关分析显示，ssPERG 幅度和幅度 D 与 ARNFLT（r≥0.53，p<0.001）、GCIPLT（r>0.38，p<0.011）和估计 RGC 计数（r>0.46，p<0.002）均呈正相关。六项中介分析表明，估计 RGC 计数介导了 ssPERG 参数、ARNFLT 和 GCIPLT 之间的关系。
稳态 PERG 参数与估计的 RGC 计数呈线性相关。ssPERG 参数与结构测量之间的关联由估计的 RGC 计数介导。
