长链非编码 RNA FOXD2-AS1 通过海绵吸附 miR-30a-3p 上调 COX-2 促进胰腺腺癌细胞侵袭和迁移。
Long Noncoding RNA FOXD2-AS1 Promotes Pancreas Adenocarcinoma Cell Invasion and Migration by Sponging miR-30a-3p to Upregulate COX-2.
机构信息
Department of Medical Oncology Center, Zhongshan People's Hospital, Zhongshan City, Guangdong Province, 528403, P.R. China.
出版信息
Crit Rev Eukaryot Gene Expr. 2022;32(1):25-33. doi: 10.1615/CritRevEukaryotGeneExpr.2021039235.
INTRODUCTION
FOXD2-AS1 is known to promote the development of several cancers. However, its role in pancreatic adenocarcinoma (PAAD) is unclear.
METHODS
Expression of FOXD2-AS1 and miR-30a-3p in PAAD patients was analyzed with RT-qPCR. A follow-up study was performed to analyze the prognostic value of FOXD2-AS1 for PAAD. Overexpression assays were performed to analyze the crosstalk between FOXD2-AS1 and miR-30a-3p. Cell invasion and migration were analyzed by transwell assays.
RESULTS
Analysis of the TCGA dataset revealed that FOXD2-AS1 was upregulated in PAAD tissues compared to the non-cancer tissues (1.89 vs. 0.2 TPM), indicating potential involvement of FOXD2-AS1 in PAAD. Our own data also showed FOXD2-AS1 was overexpressed in PAAD. Moreover, high FOXD2-AS1 levels predicted poor survival. It is predicted that miR-30a-3p can bind FOXD2-AS1, while their overexpression did not affect each other's expression. Correlation analysis revealed a significant correlation between FOXD2-AS1 and COX-2. In addition, FOXD2-AS1 overexpression increased COX-2 level, while miR-30a-3p played an opposite role. FOXD2-AS1 and COX-2 overexpression increased PAAD cell invasion and migration. MiR-30a-3p played an opposite role and inhibited the effects of FOXD2-AS1 and COX-2 overexpression.
CONCLUSION
FOXD2-AS1 may promote PAAD cell invasion and migration by sponging miR-30a-3p to upregulate COX-2.
简介
FOXD2-AS1 已知可促进多种癌症的发展。然而，其在胰腺腺癌（PAAD）中的作用尚不清楚。
方法
采用 RT-qPCR 分析 PAAD 患者中 FOXD2-AS1 和 miR-30a-3p 的表达。进行了一项随访研究，以分析 FOXD2-AS1 对 PAAD 的预后价值。通过转染实验进行了过表达实验，以分析 FOXD2-AS1 和 miR-30a-3p 之间的串扰。通过 Transwell 实验分析细胞侵袭和迁移。
结果
TCGA 数据集的分析表明，与非癌组织相比，PAAD 组织中 FOXD2-AS1 上调（1.89 比 0.2 TPM），表明 FOXD2-AS1 可能参与了 PAAD。我们自己的数据也显示 PAAD 中 FOXD2-AS1 过表达。此外，高 FOXD2-AS1 水平预示着不良的生存。预测 miR-30a-3p 可以与 FOXD2-AS1 结合，而它们的过表达并不影响彼此的表达。相关性分析显示 FOXD2-AS1 与 COX-2 之间存在显著相关性。此外，FOXD2-AS1 过表达增加了 COX-2 水平，而 miR-30a-3p 则起相反作用。FOXD2-AS1 和 COX-2 过表达增加了 PAAD 细胞的侵袭和迁移。miR-30a-3p 起相反作用，抑制了 FOXD2-AS1 和 COX-2 过表达的作用。
结论
FOXD2-AS1 可能通过海绵吸附 miR-30a-3p 来上调 COX-2，从而促进 PAAD 细胞的侵袭和迁移。
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