Department of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, Hebei, China.
Crit Rev Eukaryot Gene Expr. 2022;32(1):49-57. doi: 10.1615/CritRevEukaryotGeneExpr.2021039570.
Many recent studies have shown that microRNAs (miRNAs) in exosomes can be absorbed by nearby or distant cells, and the abnormal expression of these exosomal miRNAs is associated with most pathological progresses. In this study, we explored the diagnostic value of exosome marker proteins and exosome-derived miR-92a-3p in liver cancer. The clinicopathological data of 60 patients with liver cancer admitted to Tanghan Gongren Hospital from October 2017 to October 2019 were collected. Tumor tissue and adjacent tissue were collected during surgery. Quantitative reverse transcription polymerase chain reaction and Western blot were used to detect the expression levels of miR-92a-3p in exosomes of fibroblasts and tumor tissue, and exosome marker proteins. In liver cancer tissue and fibroblast exosomes, the expression of miR-92a-3p was significantly increased. The receiver operator characteristic curve of the expression level of miR-92a-3p in exosomes and tissue showed that the area under the curve was 0.906 and 0.911, respectively. HSP70 and CD63 were highly expressed in the tissue of liver cancer and fibroblast exosomes. miR-92a-3p was positively correlated with HSP70 and CD63 in the exosomes of liver cancer fibroblasts. In addition, miR-92a-3p and exosome marker proteins (HSP70 and CD63) were highly expressed in tumors with a diameter greater than 5 cm, and were higher in liver cancer patients with BCLC stage B/C. Tumor fibroblast-derived exosome marker proteins and miR-92a-3p have good diagnostic value in liver cancer, indicating that they may be new diagnostic markers for liver cancer.
许多最近的研究表明，外泌体中的 microRNAs（miRNAs）可以被附近或远处的细胞吸收，这些外泌体 miRNAs 的异常表达与大多数病理进展有关。在这项研究中，我们探讨了外泌体标记蛋白和外泌体来源的 miR-92a-3p 在肝癌中的诊断价值。收集了 2017 年 10 月至 2019 年 10 月期间在唐汉工人医院就诊的 60 例肝癌患者的临床病理资料。手术期间采集肿瘤组织和相邻组织。采用定量逆转录聚合酶链反应和 Western blot 检测成纤维细胞和肿瘤组织中外泌体 miR-92a-3p 及外泌体标记蛋白的表达水平。在肝癌组织和成纤维细胞外泌体中，miR-92a-3p 的表达明显增加。外泌体和组织中 miR-92a-3p 表达水平的受试者工作特征曲线显示，曲线下面积分别为 0.906 和 0.911。HSP70 和 CD63 在肝癌和成纤维细胞外泌体组织中高表达。肝癌成纤维细胞外泌体中 miR-92a-3p 与 HSP70 和 CD63 呈正相关。此外，miR-92a-3p 和外泌体标记蛋白（HSP70 和 CD63）在直径大于 5cm 的肿瘤中高表达，在 BCLC 分期 B/C 的肝癌患者中更高。肿瘤成纤维细胞衍生的外泌体标记蛋白和 miR-92a-3p 在肝癌中有良好的诊断价值，表明它们可能是肝癌的新诊断标志物。
