Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, China.
Department of Blood Transfusion, Affiliated Hospital of Nantong University, Nantong, 226001, China.
Future Oncol. 2021 Mar;17(8):907-919. doi: 10.2217/fon-2020-0792. Epub 2021 Feb 3.
Gastric cancer (GC) is one of the common malignant tumors with high mortality. The abundance of miRNAs in serum exosomes has proved to have a high application value as a new noninvasive diagnostic method. The purpose of this study was to investigate whether serum exosomal could be used as a new biomarker for early diagnosis of GC and evaluate its clinical application value by detecting the expression of serum exosomal in 131 patients with primary GC and 122 healthy controls by real-time quantitative (qRT)-PCR. The results showed that the expression level of serum exosomal in GC patients was significantly lower than that in normal controls (p < 0.0001). In addition, the level was closely correlated with lymph node metastasis and tumor node metastasis stage of GC patients. The area under the curve for serum exosomal was 0.829, significantly higher than for other indicators. Furthermore, combined detection of serum exosomal , CEA and CA19-9 was more sensitive than any of the three alone or any pair. These results showed that serum exosomal could be used as a novel new tumor biomarker to improve diagnostic efficiency in GC.
胃癌（GC）是一种常见的高死亡率恶性肿瘤。血清外泌体中 miRNA 的丰富度已被证明具有很高的应用价值，可作为一种新的非侵入性诊断方法。本研究旨在通过实时定量（qRT）-PCR 检测 131 例原发性 GC 患者和 122 例健康对照者血清外泌体中的表达，探讨血清外泌体是否可作为 GC 早期诊断的新型生物标志物，并评估其临床应用价值。结果表明，GC 患者血清外泌体中的表达水平明显低于正常对照组（p<0.0001）。此外，该水平与 GC 患者的淋巴结转移和肿瘤淋巴结转移分期密切相关。血清外泌体的曲线下面积为 0.829，明显高于其他指标。此外，血清外泌体、CEA 和 CA19-9 的联合检测比任何三种单独检测或任何两种联合检测的敏感性都更高。这些结果表明，血清外泌体可作为一种新型肿瘤标志物，提高 GC 的诊断效率。
