Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou 510275, China.
Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou 510275, China.
Bioresour Technol. 2022 May;352:127103. doi: 10.1016/j.biortech.2022.127103. Epub 2022 Apr 1.
This study investigated the temporal dynamics of digestion efficiency and community stability in digesters fed with waste activated sludge (WAS), straw (STR-AD), food waste (FW-AD) and mixture of straw-and-food waste (STR-FW-AD). Results showed that carbon removals of recalcitrant substrates (i.e., 48.2 ± 3.9% in WAS-AD and 57.8 ± 4.9% in STR-AD) were lower than that of labile substrates (i.e., 70.7 ± 4.0% in FW-AD). Nonetheless, carbon removal of recalcitrant substrates was largely improved through co-digestion (70.3 ± 3.2% in STR-FW-AD). In contrast to monopoly communities (e.g., the highly enriched Paludibacter) fed with the labile substrates, recalcitrant substrates supported highly diverse communities. Accordingly, the medians of negative/positive cohesions of communities in WAS-AD, STR-AD, STR-FW-AD and FW-AD decreased from 0.86 to 0.63, suggesting their decreasing community stability. Microbial source tracking analyses showed the major contribution of the STR-AD community to the co-digestion community. This study provided unprecedented mechanistic insight into stability improvement of substrate co-digestion on the methanogenic digestion microbiome.
本研究调查了以剩余活性污泥（WAS）、秸秆（STR-AD）、食物垃圾（FW-AD）和秸秆-食物垃圾混合物（STR-FW-AD）为食的消化器中消化效率和群落稳定性的时间动态。结果表明，难降解基质的碳去除率（WAS-AD 中的 48.2±3.9%和 STR-AD 中的 57.8±4.9%）低于易降解基质（FW-AD 中的 70.7±4.0%）。然而，难降解基质的碳去除率通过共消化得到了很大的提高（STR-FW-AD 中的 70.3±3.2%）。与以易降解基质为食的单一种群（如高度富集的 Paludibacter）不同，难降解基质支持高度多样化的群落。相应地，WAS-AD、STR-AD、STR-FW-AD 和 FW-AD 中群落负/正凝聚度的中位数从 0.86 降低到 0.63，表明其群落稳定性降低。微生物源追踪分析表明，STR-AD 群落对共消化群落的贡献最大。本研究为基质共消化对产甲烷消化微生物组稳定性提高的机制提供了前所未有的见解。
