Li Yemei, Ni Jialing, Cheng Hui, Zhu Aijun, Guo Guangze, Qin Yu, Li Yu-You
Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki-Aza, Sendai, Miyagi 980-8579, Japan.
Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki-Aza, Sendai, Miyagi 980-8579, Japan; Department of Chemical Engineering, Graduate School of Engineering, Tohoku University, 6-6-07 Aoba, Aramaki-Aza, Sendai, Miyagi 980-8579, Japan.
Bioresour Technol. 2021 Dec;342:125938. doi: 10.1016/j.biortech.2021.125938. Epub 2021 Sep 14.
The methanogenic performance and microbial community of the thermophilic anaerobic mono-digestion and co-digestion of food waste and sewage sludge in a high-solid membrane bioreactor were investigated by a continuous experiment. The methane recovery rate of the system reached 98.0% and 89.0% when the substrate was pure food waste and 25% sewage sludge substitution, respectively. Kinetics characterization showed that hydrolysis was the rate-limiting step in both mono-digestion and co-digestion while methanogenic performance and microbial community were significantly affected by feed condition. The dominant archaea for methane generation shifted from Methanothermobacter thermophilus (72.82%) to Methanosarcina thermophila (96.25%) with sewage sludge gradually added from 0% to 100% in the substrate. The relationships between digestion performance, such as the accumulation of soluble proteins in the reactor, and functional microbial groups were also carefully analyzed. Finally, reasonable metabolic pathways for mono-digestion and co-digestion were summarized.
通过连续实验研究了高固体膜生物反应器中食物垃圾与污水污泥的嗜热厌氧单消化和共消化的产甲烷性能及微生物群落。当底物分别为纯食物垃圾和25%污水污泥替代时，系统的甲烷回收率分别达到98.0%和89.0%。动力学表征表明，水解是单消化和共消化的限速步骤，而进料条件对产甲烷性能和微生物群落有显著影响。随着底物中污水污泥从0%逐渐添加到100%，产甲烷的优势古菌从嗜热栖热甲烷杆菌（72.82%）转变为嗜热嗜盐碱甲烷八叠球菌（96.25%）。还仔细分析了消化性能（如反应器中可溶性蛋白质的积累）与功能微生物群之间的关系。最后，总结了单消化和共消化的合理代谢途径。
