Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
J Chromatogr A. 2022 May 24;1671:462971. doi: 10.1016/j.chroma.2022.462971. Epub 2022 Mar 26.
Sample pre-treatment is of great significance for study protein phosphorylation and glycosylation. Due to the low concentration and suppression of matrices, the direct detection of phosphoprotein and glycoprotein by mass spectrometry (MS) are still facing many challenges. The demand of efficient and specific enrichment of phosphoprotein and glycoprotein promotes the sample preparation methods based on metal-organic frameworks (MOFs). In this review, recent advances in MOFs-based samples pre-treatment, including the enrichment of protein, endogenous peptides, glycopeptides, phosphopeptides and glycans are summarized and discussed. In addition, the design and synthesis of different types of MOFs according to different enrichment mechanisms and principles are discussed. Furthermore, the potential problems of sample pre-treatment in proteomics is prospected.
样品前处理对于研究蛋白质磷酸化和糖基化非常重要。由于浓度低和基质抑制，质谱（MS）直接检测磷酸化蛋白和糖蛋白仍然面临许多挑战。对磷酸化蛋白和糖蛋白的高效和特异性富集的需求促进了基于金属-有机框架（MOFs）的样品制备方法的发展。本综述总结并讨论了基于 MOFs 的样品前处理方法的最新进展，包括蛋白质、内源性肽、糖肽、磷酸肽和聚糖的富集。此外，还根据不同的富集机制和原理讨论了不同类型 MOFs 的设计和合成。此外，还展望了蛋白质组学中样品前处理的潜在问题。
