Department of Chemistry, The Women University, Kutchery Campus, L.M.Q. Road, Multan, 66000, Pakistan.
Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Anal Bioanal Chem. 2019 Mar;411(9):1745-1759. doi: 10.1007/s00216-019-01610-x. Epub 2019 Jan 25.
Metal-organic frameworks (MOFs) are an eminent addition to materials science research because of their versatile properties due to which their applications are wide spread in proteomics. They are used in various fields due to their characteristics like higher surface area, specific symmetry, ease of modification, and availability of a variety of ligands. As affinity sorbents, they have shown higher selectivity, sensitivity, and reproducibility than conventionally used materials. They are applied for the enrichment of phosphopeptides, glycopeptides, low mass peptides, and as laser desorption/ionization (LDI) matrices for small-molecule analysis. This review captures the insight of applying MOFs in the field of mass spectrometry-based proteomics. The specific features are discussed regarding MOFs as affinity sorbents for the selective capture of biological molecules like phosphopeptides and glycopeptides from complex samples. The potential of MOFs as LDI mass spectrometry (LDI-MS) matrices for small-molecule analysis is also evaluated. MOFs have also been used as enzymatic reactors for the digestion of proteins, prior to MS analysis. MOF-based affinity materials and bioreactors reduce proteome complexity and improve detection sensitivity and coverage. Size-exclusion effects of MOFs help in subtracting the abundant proteins in peptidomics. Several limitations of MOFs are addressed, which include stability under varying pH conditions, the unclear interaction mechanism between the MOFs and targeted analytes, and the non-specific binding that interferes during the analysis because of metal centers and ligands in the MOFs. This will open up MOF-based research to overcome the limitations and improve the performance of MOFs as selective and sensitive materials. Graphical abstract ᅟ.
金属-有机骨架（MOFs）因其多功能特性而成为材料科学研究的重要补充，其应用广泛分布于蛋白质组学领域。由于具有较高的比表面积、特定的对称性、易于修饰以及各种配体的可用性等特点，它们在各个领域得到了应用。作为亲和吸附剂，它们在选择性、灵敏度和重现性方面优于传统使用的材料。它们被应用于富集磷酸肽、糖肽、低质量肽以及作为用于小分子分析的激光解吸/电离（LDI）基质。本综述捕捉了 MOFs 在基于质谱的蛋白质组学领域的应用的洞察力。讨论了 MOFs 作为亲和吸附剂的特定特征，用于从复杂样品中选择性捕获生物分子，如磷酸肽和糖肽。还评估了 MOFs 作为 LDI 质谱（LDI-MS）基质用于小分子分析的潜力。MOFs 还被用作蛋白质的酶促反应器，然后再进行 MS 分析。基于 MOF 的亲和材料和生物反应器降低了蛋白质组的复杂性，并提高了检测灵敏度和覆盖率。MOFs 的排阻效应有助于减少肽组学中丰富的蛋白质。解决了 MOFs 的几个局限性，包括在不同 pH 条件下的稳定性、MOFs 与目标分析物之间的不清楚的相互作用机制以及由于 MOFs 中的金属中心和配体而在分析过程中干扰的非特异性结合。这将为 MOF 基研究开辟道路，以克服限制并提高 MOFs 作为选择性和灵敏材料的性能。
