Ramuta Mitchell D, Newman Christina M, Brakefield Savannah F, Stauss Miranda R, Wiseman Roger W, Kita-Yarbro Amanda, O'Connor Eli J, Dahal Neeti, Lim Ailam, Poulsen Keith P, Safdar Nasia, Marx John A, Accola Molly A, Rehrauer William M, Zimmer Julia A, Khubbar Manjeet, Beversdorf Lucas J, Boehm Emma C, Castañeda David, Rushford Clayton, Gregory Devon A, Yao Joseph D, Bhattacharyya Sanjib, Johnson Marc C, Aliota Matthew T, Friedrich Thomas C, O'Connor David H, O'Connor Shelby L
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Wisconsin National Primate Research Center, Madison, WI USA.
medRxiv. 2022 Mar 30:2022.03.29.22272716. doi: 10.1101/2022.03.29.22272716.
Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess the risk of transmission in congregate spaces. We deployed active air samplers to monitor the presence of SARS-CoV-2 in real-world settings across communities in the Upper Midwestern states of Wisconsin and Minnesota. Over 29 weeks, we collected 527 air samples from 15 congregate settings and detected 106 SARS-CoV-2 positive samples, demonstrating SARS-CoV-2 can be detected in air collected from daily and weekly sampling intervals. We expanded the utility of air surveillance to test for 40 other respiratory pathogens. Surveillance data revealed differences in timing and location of SARS-CoV-2 and influenza A virus detection in the community. In addition, we obtained SARS-CoV-2 genome sequences from air samples to identify variant lineages. Collectively, this shows air surveillance is a scalable, cost-effective, and high throughput alternative to individual testing for detecting respiratory pathogens in congregate settings.
在严重急性呼吸综合征冠状病毒2（SARS-CoV-2）出现两年后，仍然需要更好的方法来评估聚集场所的传播风险。我们部署了主动空气采样器，以监测美国中西部威斯康星州和明尼苏达州各社区实际环境中SARS-CoV-2的存在情况。在29周的时间里，我们从15个聚集场所采集了527份空气样本，检测到106份SARS-CoV-2阳性样本，这表明在每日和每周采样间隔采集的空气中都能检测到SARS-CoV-2。我们扩大了空气监测的用途，以检测另外40种呼吸道病原体。监测数据揭示了社区中SARS-CoV-2和甲型流感病毒检测时间和地点的差异。此外，我们从空气样本中获得了SARS-CoV-2基因组序列，以识别变异谱系。总体而言，这表明空气监测是一种可扩展、经济高效且高通量的替代方法，可用于在聚集场所检测呼吸道病原体的个体检测。
