Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Wisconsin National Primate Research Center, Madison, WI, USA.
Nat Commun. 2022 Aug 11;13(1):4717. doi: 10.1038/s41467-022-32406-w.
Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess the risk of transmission in congregate spaces. We deployed active air samplers to monitor the presence of SARS-CoV-2 in real-world settings across communities in the Upper Midwestern states of Wisconsin and Minnesota. Over 29 weeks, we collected 527 air samples from 15 congregate settings. We detected 106 samples that were positive for SARS-CoV-2 viral RNA, demonstrating that SARS-CoV-2 can be detected in continuous air samples collected from a variety of real-world settings. We expanded the utility of air surveillance to test for 40 other respiratory pathogens. Surveillance data revealed differences in timing and location of SARS-CoV-2 and influenza A virus detection. In addition, we obtained SARS-CoV-2 genome sequences from air samples to identify variant lineages. Collectively, this shows air sampling is a scalable, high throughput surveillance tool that could be used in conjunction with other methods for detecting respiratory pathogens in congregate settings.
SARS-CoV-2 出现两年后，仍需要更好的方法来评估聚集场所的传播风险。我们部署了主动空气采样器，以监测威斯康星州和明尼苏达州上中西部各州社区内真实环境中 SARS-CoV-2 的存在情况。在 29 周的时间里，我们从 15 个聚集场所采集了 527 个空气样本。我们检测到 106 个 SARS-CoV-2 病毒 RNA 阳性样本，表明可以从各种真实环境中采集的连续空气样本中检测到 SARS-CoV-2。我们扩展了空气监测的用途，以检测其他 40 种呼吸道病原体。监测数据显示了 SARS-CoV-2 和甲型流感病毒检测的时间和地点的差异。此外，我们从空气样本中获得了 SARS-CoV-2 基因组序列，以鉴定变异谱系。总的来说，这表明空气采样是一种可扩展的高通量监测工具，可以与其他方法结合使用，以检测聚集场所中的呼吸道病原体。
