Huang Pu, Liu Yawen, Jia Baoqing
Medical School of Chinese PLA, Beijing, China.
Department of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, China.
J Oncol. 2022 Mar 26;2022:8167496. doi: 10.1155/2022/8167496. eCollection 2022.
Gastric cancer (GC) is a lethal cancer with a poor 5-year relative survival, which requires a new research perspective. Our study aims to explore the biological impact of the mast cell-expressed membrane protein 1 (MCEMP1) in GC, which includes its expression and potential biological functions.
The expression of MCEMP1 was assessed through public databases. The GO, KEGG, and GESA analyses were conducted to explore the biofunction of MCEMP1. And ssGSEA was used to analyze the infiltration of the immune cells for MCEMP1. The proliferation, migration, and invasion of GC cells were analyzed through CCK8, colony-forming, wound healing, Transwell, and Western blot assay.
The expression of MCEMP1 was higher in GC tissues. Further, we found a close relationship between MCEMP1 and poorer prognosis of gastric cancer by prognostic analysis. The functional analysis showed that MCEMP1 is involved in immune, inflammation, and metabolism-related pathways. The ssGSEA analysis indicated MCEMP1 mRNA expression was associated with immune infiltration of multiple immune cells. In cellular experiments, the invasion and metastasis of gastric cancer cells could be promoted by regulating the rise of MCEMP1 expression. Western blot analysis showed that regulation of MCEMP1 expression can affect EMT-related protein expression and that NF-B expression is involved in this process.
MCEMP1 shows a potential value for the prognosis in GC. And, abnormal expression of MCEMP1 in GC is correlated with tumor immune cell infiltration. In in vitro experiments, MCEMP1 can affect the proliferation, migration, and invasion of GC cells by regulating EMT, in which TLR4/NOD2/NF-B was involved.
胃癌（GC）是一种致死性癌症，5年相对生存率较低，需要新的研究视角。我们的研究旨在探讨肥大细胞表达的膜蛋白1（MCEMP1）在胃癌中的生物学影响，包括其表达及潜在生物学功能。
通过公共数据库评估MCEMP1的表达。进行基因本体（GO）、京都基因与基因组百科全书（KEGG）和基因集富集分析（GESA）以探索MCEMP1的生物功能。采用单样本基因集富集分析（ssGSEA）分析MCEMP1的免疫细胞浸润情况。通过细胞计数试剂盒8（CCK8）、集落形成、伤口愈合、Transwell和蛋白质免疫印迹法检测分析胃癌细胞的增殖、迁移和侵袭能力。
MCEMP1在胃癌组织中的表达较高。此外，通过预后分析，我们发现MCEMP1与胃癌预后较差密切相关。功能分析表明，MCEMP1参与免疫、炎症和代谢相关通路。ssGSEA分析表明MCEMP1 mRNA表达与多种免疫细胞的免疫浸润相关。在细胞实验中，上调MCEMP1表达可促进胃癌细胞的侵袭和转移。蛋白质免疫印迹分析表明，调节MCEMP1表达可影响上皮-间质转化（EMT）相关蛋白表达，且核因子-κB（NF-κB）表达参与此过程。
MCEMP1在胃癌预后中显示出潜在价值。并且，MCEMP1在胃癌中的异常表达与肿瘤免疫细胞浸润相关。在体外实验中，MCEMP1可通过调节EMT影响胃癌细胞的增殖、迁移和侵袭，此过程涉及Toll样受体4（TLR4）/核苷酸结合寡聚化结构域蛋白2（NOD2）/NF-κB。
