Chen Xiaojing, Hu Lanxin, Mao Xinrui, Chen Haoran, She Yuchen, Chi Hao, Zeng Hao, Guo Lu, Han Yunwei
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
Department of Clinical Medicine, Southwest Medical University, Luzhou, China.
J Oncol. 2022 Apr 11;2022:1608936. doi: 10.1155/2022/1608936. eCollection 2022.
LINC00922 has been found to promote epithelial-mesenchymal transition (EMT) in a variety of tumors. But its functions in gastric cancer (GC) remain unclear. We attempt to investigate the correlation between LINC00922 and GC via bioinformatics analysis, in vitro and in vivo experiments.
TCGA and GTEx databases were utilized to obtain the RNAseq and clinical data of GC, and then, identified the correlation of LINC00922 with patients' clinicopathological characteristics and prognosis. GSEA and GO/KEGG enrichment analyses were performed to explore the potential functions or signaling pathways that LINC00922 participated in GC. Infiltration levels of immune cells were employed by ssGSEA algorithm, and then Wilcoxon rank sum test was applied to analyze their correlations with LINC00922. Scratch and transwell assays were conducted to detect the invasion and migration abilities of GC cells. Western blot was performed to explore the expression level of EMT-related proteins. Furthermore, we constructed the xenograft tumor model and metastatic tumor model in nude mice to explore the effect of LINC00922 downregulating on metastasis of GC cells in vivo.
Compared with normal tissues, LINC00922 was highly expressed in GC tissues and positively correlated with poor prognosis. The correlation existed between LINC00922 and immune infiltration in GC. Downregulation of LINC00922 inhibited the EMT process of GC cells. In addition, both in vitro and in vivo experiments showed that LINC00922 affects the invasion and migration abilities of GC.
LINC00922 promotes the migration, invasion, and EMT in GC and has the potential to be used as a prognostic biomarker and therapeutic target for GC.
已发现LINC00922在多种肿瘤中促进上皮-间质转化（EMT）。但其在胃癌（GC）中的功能仍不清楚。我们试图通过生物信息学分析、体外和体内实验研究LINC00922与GC之间的相关性。
利用TCGA和GTEx数据库获取GC的RNA测序和临床数据，然后确定LINC00922与患者临床病理特征及预后的相关性。进行基因集富集分析（GSEA）和基因本体论（GO）/京都基因与基因组百科全书（KEGG）富集分析，以探索LINC00922参与GC的潜在功能或信号通路。采用单样本基因集富集分析（ssGSEA）算法计算免疫细胞浸润水平，然后应用Wilcoxon秩和检验分析其与LINC00922的相关性。进行划痕实验和Transwell实验以检测GC细胞的侵袭和迁移能力。进行蛋白质免疫印迹法以探究EMT相关蛋白的表达水平。此外，我们在裸鼠中构建了异种移植瘤模型和转移瘤模型，以探究下调LINC00922对GC细胞体内转移的影响。
与正常组织相比，LINC00922在GC组织中高表达，且与不良预后呈正相关。LINC00922与GC中的免疫浸润存在相关性。下调LINC00922可抑制GC细胞的EMT过程。此外，体外和体内实验均表明，LINC00922影响GC的侵袭和迁移能力。
LINC00922促进GC的迁移、侵袭和EMT，有潜力作为GC的预后生物标志物和治疗靶点。
