Laboratory for Comparative Genomics and Bioinformatics and Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, People's Republic of China; and.
Jiangsu Provincial Key Construction Laboratory of Special Biomass Byproduct Resource Utilization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, People's Republic of China.
J Immunol. 2022 Apr 15;208(8):1978-1988. doi: 10.4049/jimmunol.2100658. Epub 2022 Apr 4.
The Toll signaling pathway mainly responds to Gram-positive (G) bacteria or fungal infection, which is highly conserved with mammalian TLR signaling pathway. Although many positive and negative regulators involved in the immune response of the Toll pathway have been identified in , the roles of long noncoding RNAs (lncRNAs) in Toll immune responses are poorly understood to date. In this study, our results demonstrate that is mainly expressed in the nucleus and upregulated after infection. Especially, not only modulates differential expressions of multiple antimicrobial peptide genes but also affects the survival rate during response to G bacterial infection based on the transiently overexpressing and the knockdown assays in vivo. Mechanically, interacts with the NF-κB transcription factors Dorsal-related immunity factor/Dorsal to promote the transcriptions of antimicrobial peptides and , thus enhancing Toll immune responses. Taken together, this study identifies as a positive regulator of innate immune response to G bacterial infection to facilitate Toll signaling via interacting with Dorsal-related immunity factor/Dorsal. It would be helpful to reveal the roles of lncRNAs in Toll immune response in and provide insights into animal innate immunity.
Toll 信号通路主要响应革兰氏阳性（G）细菌或真菌感染，与哺乳动物 TLR 信号通路高度保守。虽然在 中已经鉴定出许多参与 Toll 通路免疫反应的正调控因子和负调控因子，但长链非编码 RNA（lncRNA）在 Toll 免疫反应中的作用迄今知之甚少。在本研究中，我们的结果表明， 在 感染后主要在核内表达并上调。特别是， 通过体内瞬时过表达和敲低实验， 不仅调节多种抗菌肽基因的差异表达，而且影响 G 细菌感染时的 存活率。在机制上， 通过与 NF-κB 转录因子 Dorsal-related immunity factor/Dorsal 相互作用，促进抗菌肽 和 的转录，从而增强 Toll 免疫反应。总之，本研究鉴定了 作为 G 细菌感染先天免疫反应的正调控因子，通过与 Dorsal-related immunity factor/Dorsal 相互作用促进 Toll 信号转导。这有助于揭示 lncRNA 在 中 Toll 免疫反应中的作用，并为动物先天免疫提供新的见解。
