Ren Yipeng, Fu Siying, Dong Wenhao, Chen Juhong, Xue Huaijun, Bu Wenjun
Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.
Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China.
Front Microbiol. 2024 May 17;15:1386345. doi: 10.3389/fmicb.2024.1386345. eCollection 2024.
Insects depend on humoral immunity against intruders through the secretion of antimicrobial peptides (AMPs) and immune effectors via NF-κB transcription factors, and their fitness is improved by gut bacterial microbiota. Although there are growing numbers of reports on noncoding RNAs (ncRNAs) involving in immune responses against pathogens, comprehensive studies of ncRNA-AMP regulatory networks in , which is one of the widely distributed pests in East Asia, are still not well understood under feeding environmental changes. The objective of this study employed the whole-transcriptome sequencing (WTS) to systematically identify the lncRNAs (long noncoding RNA) and circRNAs (circular RNA) and to obtain their differential expression from the gut under different feeding conditions. Functional annotation indicated that they were mainly enriched in various biological processes with the GO and KEGG databases, especially in immune signaling pathways. Five (four novel members) and eleven (nine novel members) family genes were identified and characterized from WTS data, and meanwhile, phylogenetic analysis confirmed their classification. Subsequently, the miRNA-mRNA interaction network of above two AMPs and lncRNA-involved ceRNA (competing endogenous RNA) regulatory network of one were predicted and built based on bioinformatic prediction and calculation, and the expression patterns of differentially expressed (DE) , and DE and related DE ncRNAs were estimated and selected among all the comparison groups. Finally, to integrate the analyses of WTS and previous 16S rRNA amplicon sequencing, we conducted the Pearson correlation analysis to reveal the significantly positive or negative correlation between above DE AMPs and ncRNAs, as well as most changes in the gut bacterial microbiota at the genus level of . Taken together, the present observations provide great insights into the ncRNA regulatory networks of AMPs in response to rearing environmental changes in insects and uncover new potential strategies for pest control in the future.
昆虫通过经由核因子κB转录因子分泌抗菌肽（AMPs）和免疫效应因子来依靠体液免疫抵御入侵者，并且其健康状况会因肠道细菌微生物群而得到改善。尽管关于非编码RNA（ncRNAs）参与针对病原体的免疫反应的报道越来越多，但在东亚广泛分布的害虫之一——[此处原文缺失具体害虫名称]中，在饲养环境变化下对ncRNA-AMP调控网络的全面研究仍未得到充分了解。本研究的目的是利用全转录组测序（WTS）系统地鉴定长链非编码RNA（lncRNAs）和环状RNA（circRNAs），并在不同饲养条件下从[此处原文缺失具体组织名称]肠道中获得它们的差异表达。功能注释表明，它们主要通过GO和KEGG数据库富集于各种生物学过程，尤其是免疫信号通路。从WTS数据中鉴定并表征了五个[此处原文缺失具体基因家族名称]（四个新成员）和十一个[此处原文缺失具体基因家族名称]（九个新成员）家族基因，同时，系统发育分析证实了它们的分类。随后，基于生物信息学预测和计算，预测并构建了上述两种AMPs的miRNA-mRNA相互作用网络以及一种[此处原文缺失具体基因名称]的lncRNA参与的ceRNA（竞争性内源RNA）调控网络，并在所有比较组中估计并选择了差异表达（DE）[此处原文缺失具体基因名称]、DE[此处原文缺失具体基因名称]和相关DE ncRNAs的表达模式。最后，为了整合WTS分析和先前的16S rRNA扩增子测序，我们进行了Pearson相关分析，以揭示上述DE AMPs和ncRNAs之间以及[此处原文缺失具体害虫名称]属水平上肠道细菌微生物群的大多数变化之间的显著正相关或负相关。综上所述，本研究结果为昆虫中AMPs的ncRNA调控网络响应饲养环境变化提供了深刻见解，并揭示了未来害虫防治的新潜在策略。
