Institute of Zoology, University of Cologne, Cologne, Germany.
J Endocrinol. 2022 May 10;254(1):1-12. doi: 10.1530/JOE-22-0026.
Loss of LEPR function (LOF) in mammals leads to diverse phenotypes including morbid obesity and infertility while zebrafish show relatively minor phenotypes. This however allows the study of LEPR LOF in the absence of the detrimental effects of hyperglycemia or obesity. Here, we show evidence that leptin plays a role in the central as well as peripheral regulation of the hypothalamic-pituitary-gonadal (HPG) axis in zebrafish. Animals with a Lepr LOF show dysregulated pituitary HPG genes as well as evidence that oocytes mature slower and/or exhibit an increased rate of atresia. In culture, Lepr LOF attenuates the effect of 17α-20β-dihydroxy-4 pregnen-3-one in promoting germinal vesicle breakdown (GVBD) and increases the rate of GVBD as well as attenuates the rate of oocyte atresia.
哺乳动物 LEPR 功能丧失（LOF）会导致多种表型，包括病态肥胖和不育，而斑马鱼则表现出相对较小的表型。然而，这使得在没有高血糖或肥胖等不利影响的情况下研究 LEPR LOF 成为可能。在这里，我们证明了瘦素在斑马鱼的中枢和外周调节下丘脑-垂体-性腺（HPG）轴中发挥作用。Lepr LOF 的动物表现出垂体 HPG 基因失调的现象，以及卵母细胞成熟较慢和/或闭锁率增加的证据。在培养中，Lepr LOF 减弱了 17α-20β-二羟孕酮-4 烯-3-酮促进生发泡破裂（GVBD）的作用，并增加了 GVBD 的速率，同时减弱了卵母细胞闭锁的速率。
