Department of Biology, University of Ottawa, Ottawa, ON, Canada.
Department of Physiological Sciences, University of Florida, Gainesville, FL, United States.
Front Endocrinol (Lausanne). 2023 Oct 9;14:1266985. doi: 10.3389/fendo.2023.1266985. eCollection 2023.
The detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using different extraction methodologies. With the new advancement on HPLC sorbents, the simultaneous measurement of hormones from different categories becomes possible. In this study, we present a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins using high-resolution liquid chromatography tandem mass spectrometry. We demonstrate the sensitivity of our method for small tissues by acquiring data from brain, pituitary gland, and gonads of single zebrafish samples. This approach promises to shed light on the hormonal pathways and their interrelationships, providing knowledge on the integration of hormone systems.
激素的检测和定量对于评估实验模型的生殖和应激状态以及人类和兽医临床疾病的诊断非常重要。传统上，甾体、肽和蛋白质激素是在单独的实验中使用不同的提取方法进行分析的。随着 HPLC 吸附剂的新进展，来自不同类别的激素的同时测量成为可能。在这项研究中，我们提出了一种新的样品处理策略，用于使用高分辨率液相色谱串联质谱同时提取和检测肽、甾体和蛋白质。我们通过从单个斑马鱼样本的大脑、垂体和性腺中获取数据，证明了我们的方法对小组织的灵敏度。这种方法有望揭示激素途径及其相互关系，提供关于激素系统整合的知识。
