Sternberger N H, Itoyama Y, Kies M W, Webster H D
Proc Natl Acad Sci U S A. 1978 May;75(5):2521-4. doi: 10.1073/pnas.75.5.2521.
A specific antibody to myelin basic protein has been used to localize the protein in developing rat oligodendroglia and myelin. Basic protein is found in the oligodendroglial cytoplasm of anterior commissures of 5- and 7-day old rats before the beginning of myelination. Staining of basic protein in oligodendroglia increases, becoming most intense during early myelination; it decreases during rapid myelination. Staining intensity of oligodendroglia is dependent upon age, brain region, and nervous tract studied. In myelin, reaction of basic protein with antibody decreases when large compact sheaths are present, unless tissue sections are first treated with alcohol.
一种针对髓鞘碱性蛋白的特异性抗体已被用于在发育中的大鼠少突胶质细胞和髓鞘中定位该蛋白。在髓鞘形成开始前，在5日龄和7日龄大鼠前连合的少突胶质细胞质中发现了碱性蛋白。少突胶质细胞中碱性蛋白的染色增加，在早期髓鞘形成期间变得最为强烈；在快速髓鞘形成期间则减少。少突胶质细胞的染色强度取决于所研究的年龄、脑区和神经束。在髓鞘中，当存在大的致密髓鞘时，碱性蛋白与抗体的反应会降低，除非组织切片先用酒精处理。
