Regional Medical Research Centre (ICMR), Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha, India.
Regional Medical Research Centre (ICMR), Nalco Square, Chandrasekharpur; KIIT School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, Odisha, India.
J Vector Borne Dis. 2021 Oct-Dec;58(4):297-305. doi: 10.4103/0972-9062.313967.
Wolbachia, known for its reproductive manipulation capabilities in insects, are being implemented to control dengue and chikungunya. To understand Wolbachia biology and its utility as a bio-control for vector mosquito's populations, we investigated its dissemination pattern in field in collected Ae. albopictus along with its maternal transmission efficacy over generations in regions of endemic dengue (DENV) transmission. Field collected Ae. albopictus were subjected to PCR for Wolbachia screening. Overall mean Wolbachia infection frequency in Ae. albopictus was found out to be 87.3% wherein a trend was observed in the pattern of maternal transmission across generations. χ for trend revealed a significant variation between Wolbachia infections and non-infections in Ae. albopictus generations. Linear regression analysis revealed the involvement of a strong negative correlation, implying that overall Wolbachia infection tends to decrease in places with high dengue cases.The reduction in Wolbachia infection frequency may be attributed to several environmental factors with the probability of being the cause for endemicity of dengue in the studied areas.This study reports on the transmission efficacy of naturally occurring Wolbachia in successive generations of Ae. albopictus and its correlation with dengue cases in clusters of Odisha, India. Studying the transmission trend of Wolbachia along with transovarial transmission of DENV might be indicative towards the interplay of Wolbachia infection in presence/absence of DENV.
沃尔巴克氏体（Wolbachia）以其在昆虫中的生殖操纵能力而闻名，目前正被用于控制登革热和基孔肯雅热。为了了解沃尔巴克氏体的生物学特性及其作为病媒蚊种群生物控制的用途，我们研究了其在收集的白纹伊蚊（Aedes albopictus）中的野外传播模式，以及其在登革热（DENV）传播地区的世代间母体传播效率。采集的白纹伊蚊样本进行了沃尔巴克氏体的 PCR 筛选。研究发现，白纹伊蚊的沃尔巴克氏体总体感染频率为 87.3%，在跨世代的母体传播模式中观察到了一种趋势。趋势的 χ 检验揭示了白纹伊蚊世代中沃尔巴克氏体感染和非感染之间存在显著差异。线性回归分析显示，存在强烈的负相关，这意味着在登革热病例较多的地方，沃尔巴克氏体的总体感染倾向于减少。沃尔巴克氏体感染频率的降低可能归因于多种环境因素，这些因素可能是导致研究地区登革热地方性流行的原因。本研究报告了自然发生的沃尔巴克氏体在白纹伊蚊连续世代中的传播效率及其与印度奥里萨邦集群中登革热病例的相关性。研究沃尔巴克氏体的传播趋势以及 DENV 的经卵传播可能有助于了解沃尔巴克氏体感染与 DENV 存在/不存在的相互作用。
