Joanne Sylvia, Vythilingam Indra, Yugavathy Nava, Leong Cherng-Shii, Wong Meng-Li, AbuBakar Sazaly
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Acta Trop. 2015 Aug;148:38-45. doi: 10.1016/j.actatropica.2015.04.003. Epub 2015 Apr 18.
Wolbachia are maternally transmitted bacteria found in most arthropods and nematodes, but little is known about their distribution and reproductive dynamics in the Malaysian dengue vector Aedes albopictus. In this study, polymerase chain reaction (PCR) was used to determine the presence of Wolbachia from field collected Ae. albopictus from various parts of the country using wsp specific primers. Ae. albopictus had Wolbachia infection ranging from 60 to 100%. No sequence diversity of wsp gene was found within all wAlbA and wAlbB sequences. Our findings suggest that Wolbachia infection amongst the Malaysian Ae. albopictus were not homogenously distributed in all districts in Malaysia. The presence of Wolbachia in different organs of Ae. albopictus was also determined. Wolbachia were only found in the ovaries and midguts of the mosquitoes, while absent in the salivary glands. The effects of Wolbachia on Ae. albopictus fecundity, longevity and egg viability were studied using infected and uninfected colonies. The removal of Wolbachia from Ae. albopictus resulted in reduced fecundity, longevity and egg viability, thus. Wolbachia seem to play a vital role in Ae. albopictus reproductive system.
沃尔巴克氏体是一种通过母体传播的细菌，存在于大多数节肢动物和线虫中，但人们对其在马来西亚登革热传播媒介白纹伊蚊中的分布和繁殖动态知之甚少。在本研究中，采用聚合酶链反应（PCR），使用 wsp 特异性引物，从该国各地野外采集的白纹伊蚊中检测沃尔巴克氏体的存在情况。白纹伊蚊的沃尔巴克氏体感染率在 60%至 100%之间。在所有 wAlbA 和 wAlbB 序列中未发现 wsp 基因的序列多样性。我们的研究结果表明，马来西亚白纹伊蚊中的沃尔巴克氏体感染在马来西亚的所有地区分布并不均匀。还确定了沃尔巴克氏体在白纹伊蚊不同器官中的存在情况。沃尔巴克氏体仅在蚊子的卵巢和中肠中发现，而在唾液腺中不存在。使用感染和未感染的蚊群研究了沃尔巴克氏体对白纹伊蚊繁殖力、寿命和卵活力的影响。从白纹伊蚊中去除沃尔巴克氏体导致繁殖力、寿命和卵活力降低。因此，沃尔巴克氏体似乎在白纹伊蚊的生殖系统中起着至关重要的作用。
