苏丹内脏利什曼病和黏膜利什曼病的血清学诊断：酶联免疫吸附测定-免疫荧光法与间接血凝试验的比较
Serodiagnosis of Sudanese visceral and mucosal leishmaniasis: comparison of ELISA--immunofluorescence and indirect haemagglutination.
作者信息
el Amin E R, Wright E P, Abdel Rahman A M, Kolk A, Laarman J J, Pondman K W
出版信息
Trans R Soc Trop Med Hyg. 1986;80(2):271-4. doi: 10.1016/0035-9203(86)90033-7.
PMID:3538523
Abstract
Two established immunodiagnostic techniques, immunofluorescence and indirect haemagglutination, were compared with ELISA (enzyme-linked immunosorbent assay) using intact promastigotes as antigen for the detection of specific antibodies against Leishmania in the serum of patients with visceral or mucosal leishmaniasis from the Sudan. The ELISA was found to be more sensitive and more specific than either of the other two tests.
摘要
将两种成熟的免疫诊断技术，即免疫荧光和间接血凝，与酶联免疫吸附测定法（ELISA）进行了比较，使用完整的前鞭毛体作为抗原，以检测来自苏丹的内脏利什曼病或黏膜利什曼病患者血清中针对利什曼原虫的特异性抗体。结果发现，ELISA比其他两种检测方法中的任何一种都更敏感、更特异。
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