Institute of Medical Microbiology and Virology, University Hospital Carl Gustav Carus, Medical Faculty, Technische Universität Dresden, Dresden, Germany.
J Clin Microbiol. 2022 May 18;60(5):e0253321. doi: 10.1128/jcm.02533-21. Epub 2022 Apr 7.
Time to results for identification (ID) and antimicrobial susceptibility testing (AST) from blood cultures is an important factor impacting outcome in sepsis. In this study we evaluated a novel device, the FAST™ system from Qvella that concentrates microbial biomass from positive blood culture flasks with the FAST-PBC Prep™ cartridge thereby producing a liquid colony™ (LC), which can be used immediately in standard laboratory downstream applications. We tested 250 positive blood culture bottles collected from January 2021 to May 2021. Results were obtained either with LC or from bacterial overnight cultures using Bruker's MALDI Biotyper™ and bioMérieux's Vitek 2. We compared ID and AST results obtained by both methods and evaluated turnaround times. Two-hundred and fourteen blood cultures could be included in the analysis. In 94% of the cases ( = 201) identification was obtained directly from the LC with concordant results compared to the standard workflow. No discordant results were observed. AST results could be analyzed for 175 samples. Using categorical analysis, concordant agreement was 97.4% of 1,676 AST results for Gram positive bacteria. Agreement for Gram negative bacteria was 98.5% of 980 AST results. Times-to-result were 36.9 h versus 12.8 h for ID and 52.9 h versus 26.8 h for AST in routine workflow vs FAST system, respectively. The FAST system gives reliable results for ID and AST directly from positive blood cultures and allows for significant time savings in blood culture diagnostics.
从血培养物中获得鉴定（ID）和抗菌药物敏感性测试（AST）的结果时间是影响脓毒症患者结局的一个重要因素。在这项研究中，我们评估了 Qvella 的新型设备 FAST™系统，该系统使用 FAST-PBC Prep™卡从阳性血培养瓶中浓缩微生物生物量，从而产生液体集落™（LC），可立即用于标准实验室下游应用。我们测试了 2021 年 1 月至 2021 年 5 月期间收集的 250 个阳性血培养瓶。结果要么通过 LC 获得，要么通过 Bruker 的 MALDI Biotyper™和 bioMérieux 的 Vitek 2 从细菌过夜培养物中获得。我们比较了两种方法获得的 ID 和 AST 结果，并评估了周转时间。可将 214 个血培养物纳入分析。在 94%的情况下（ = 201），与标准工作流程相比，直接从 LC 获得了鉴定结果，结果一致。未观察到不一致的结果。可以分析 175 个样本的 AST 结果。使用分类分析，对于 1,676 个革兰氏阳性菌的 AST 结果，一致性为 97.4%。对于 980 个革兰氏阴性菌的 AST 结果，一致性为 98.5%。在常规工作流程与 FAST 系统中，ID 的结果时间分别为 36.9 小时和 12.8 小时，AST 的结果时间分别为 52.9 小时和 26.8 小时。FAST 系统可直接从阳性血培养物中获得可靠的 ID 和 AST 结果，并可显著节省血培养诊断的时间。
