University of California San Diego, Department of Pathology, San Diego, CA, USA.
University of California San Diego, Department of Pathology, San Diego, CA, USA; University of California San Diego, Department of Medicine, La Jolla, CA, USA.
Diagn Microbiol Infect Dis. 2022 Dec;104(4):115783. doi: 10.1016/j.diagmicrobio.2022.115783. Epub 2022 Jul 30.
The high morbidity and mortality of sepsis can be impacted by expediting identification (ID) and antibiotic susceptibility testing (AST) of causative bacteria. We evaluated the Qvella FAST™ System which creates a Liquid Colony™ (LC) from blood cultures that can be used to expedite results by 24 to 48 hours. We analyzed 289 LC samples and found that there were 17 (5.9%) that resulted in no ID. One hundred percent of the LC samples that produced an ID were concordant with SOC identification. Gram-positive bacteria showed a categorical agreement (CA) of 99.5%, with 3 minor errors (minE), and no major errors (majE) or very major errors (VME), and essential agreement (EA) of 98.9%. For Gram-negatives, the CA was 97.8% and the EA was 98.5% with 31 minE, 0 majE, and 2 VME. The FAST-System™ can accelerate ID and AST by 24 to 48 hours with potential positive impacts on time to effective therapy for sepsis.
脓毒症的高发病率和死亡率可以通过加快鉴定（ID）和抗生素药敏试验（AST）来影响。我们评估了 Qvella FAST 系统，该系统从血液培养物中创建液体集落（LC），可以将结果提前 24 至 48 小时。我们分析了 289 个 LC 样本，发现有 17 个（5.9%）没有鉴定结果。产生 ID 的 LC 样本 100%与 SOC 鉴定一致。革兰氏阳性菌的分类一致性（CA）为 99.5%，有 3 个小错误（minE），没有大错误（majE）或非常大错误（VME），基本一致（EA）为 98.9%。对于革兰氏阴性菌，CA 为 97.8%，EA 为 98.5%，有 31 个 minE，0 个 majE，2 个 VME。FAST 系统可以将 ID 和 AST 提前 24 至 48 小时，有可能对脓毒症的有效治疗时间产生积极影响。
