HOXA-AS2 通过抑制 miR-2116-3p 从而上调 SERPINA3 来增强 GBM 细胞的恶性程度。
HOXA-AS2 enhances GBM cell malignancy by suppressing miR-2116-3p thereby upregulating SERPINA3.
机构信息
Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan, China.
Information Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
出版信息
BMC Cancer. 2022 Apr 6;22(1):366. doi: 10.1186/s12885-022-09462-y.
BACKGROUND
Glioblastoma (GBM) is malignant, demanding more attention to the improvement of the diagnosis and therapy. LncRNAs have been implicated in the malignancy of GBM cells.
METHODS
HOXA-AS2, miR-2116-3p and SERPINA3 expression levels in GBM tissues and cell lines were detected by qRT-PCR. Western blotting was performed to detect the protein levels of Bax and Bcl-2. Dual-luciferase reporter assay was for detection of relationship among these factors, together with RIP and RNA pull-down. CCK-8, EdU, wound healing and transwell assays were for detection of the role of HOXA-AS2, miR-2116-3p and SERPINA3 in cell viability, proliferation, migration and invasion in GBM, respectively.
RESULTS
HOXA-AS2 and SERPINA3 showed higher level in GBM tissues and cell lines. Low level of HOXA-AS2 attenuated GBM cell growth in vitro. Moreover, the anti-tumor impact of silenced HOXA-AS2 was restored by miR-2116-3p inhibitor, but its tumor-promotional effect could be reversed by silenced SERPINA3.
CONCLUSION
HOXA-AS2 enhanced GBM cell malignancy through sponging miR-2116-3p and releasing SERPINA3, which might shed light on the diagnosis and therapy for GBM in the future.
背景
胶质母细胞瘤（GBM）是恶性的，需要更多地关注其诊断和治疗的改善。lncRNAs 已被牵涉到 GBM 细胞的恶性中。
方法
通过 qRT-PCR 检测 GBM 组织和细胞系中 HOXA-AS2、miR-2116-3p 和 SERPINA3 的表达水平。通过 Western blot 检测 Bax 和 Bcl-2 的蛋白水平。双荧光素酶报告实验用于检测这些因素之间的关系，以及 RIP 和 RNA 下拉实验。CCK-8、EdU、划痕愈合和 Transwell 实验分别用于检测 HOXA-AS2、miR-2116-3p 和 SERPINA3 在 GBM 细胞活力、增殖、迁移和侵袭中的作用。
结果
HOXA-AS2 和 SERPINA3 在 GBM 组织和细胞系中表达水平较高。HOXA-AS2 水平降低可减弱 GBM 细胞的体外生长。此外，沉默 HOXA-AS2 的抗肿瘤作用可被 miR-2116-3p 抑制剂恢复，但沉默 SERPINA3 可逆转其促肿瘤作用。
结论
HOXA-AS2 通过海绵吸附 miR-2116-3p 并释放 SERPINA3 增强了 GBM 细胞的恶性程度，这可能为未来 GBM 的诊断和治疗提供新的思路。
Zotero 插件