Hoffmann S, Henrichsen J
Acta Pathol Microbiol Immunol Scand B. 1986 Oct;94(5):347-50. doi: 10.1111/j.1699-0463.1986.tb03065.x.
The relative sensitivities of four commercially available kits for the demonstration of the group antigen of group A streptococcus were estimated in laboratory experiments. Two kits gave positive reactions with swabs charged with approximately 10(5) colony-forming units of group A hemolytic streptococci of five common T-types. The two other kits required inocula ten times higher. Application of the antigen detection reagents from each kit to antigen extracts prepared by extraction reagents from the other kits revealed that the differences in sensitivity were largely attributable to differences in the extraction abilities. The four kits did not differ appreciably in their ability to demonstrate group A antigen in human pus mixed with group A streptococci; the minimum inoculum detectable was approximately 10(6) colony-forming units per 0.04 ml of pus.
在实验室实验中评估了四种市售试剂盒用于检测A组链球菌群抗原的相对敏感性。两种试剂盒对含有五种常见T型A组溶血性链球菌约10⁵菌落形成单位的拭子呈阳性反应。另外两种试剂盒所需的接种量要高十倍。将每个试剂盒的抗原检测试剂应用于由其他试剂盒的提取试剂制备的抗原提取物，结果表明敏感性差异很大程度上归因于提取能力的差异。这四种试剂盒在检测与人A组链球菌混合的人脓液中的A组抗原的能力上没有明显差异；每0.04 ml脓液中可检测到的最小接种量约为10⁶菌落形成单位。
