Kofod H, Lernmark A, Hedeskov C J
Acta Physiol Scand. 1986 Nov;128(3):335-40. doi: 10.1111/j.1748-1716.1986.tb07986.x.
Column perifusion of mouse pancreatic islets was used to study the ability of amino acids and their methyl esters to influence insulin release and activate islet glutamate dehydrogenase activity. In the absence of L-glutamine, L-serine and the methyl ester of L-phenylalanine, but neither L-phenylalanine nor L-serine methyl ester, stimulate insulin secretion. In the presence of L-glutamine, however, the effect of L-serine was additive, while the methyl esters of L-serine and L-phenylalanine as well as native L-phenylalanine, potentiated the glucose-stimulated release of insulin. Measurements of islet glutamate dehydrogenase activity showed that only the two methyl esters of L-phenylalanine and L-serine activated the enzyme. It is concluded that the mechanism by which methyl esters of amino acids potentiate insulin release is most likely to be mediated by the activation of pancreatic beta-cell glutamate dehydrogenase activity.
采用小鼠胰岛柱灌注法研究氨基酸及其甲酯影响胰岛素释放和激活胰岛谷氨酸脱氢酶活性的能力。在缺乏L-谷氨酰胺、L-丝氨酸和L-苯丙氨酸甲酯的情况下，L-苯丙氨酸和L-丝氨酸甲酯均不刺激胰岛素分泌，但L-丝氨酸可刺激胰岛素分泌。然而，在有L-谷氨酰胺存在时，L-丝氨酸的作用是相加的，而L-丝氨酸甲酯、L-苯丙氨酸甲酯以及天然的L-苯丙氨酸均可增强葡萄糖刺激的胰岛素释放。胰岛谷氨酸脱氢酶活性测定表明，只有L-苯丙氨酸和L-丝氨酸的两种甲酯可激活该酶。得出的结论是，氨基酸甲酯增强胰岛素释放的机制很可能是通过激活胰腺β细胞谷氨酸脱氢酶活性介导的。
